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Pannexin-1 influences peritoneal cavity cell population but is not involved in NLRP3 inflammasome activation |
Hongbin Wang1, Yue Xing1, Liming Mao1, Yi Luo2, Lishan Kang2, Guangxun Meng1( ) |
| 1. 1Unit of Innate Immunity, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai 200025, China; 2. Beijing Novo Nordisk Pharmaceuticals Science and Technology Co., Ltd., 29 Life Science Park Road, Beijing 102206, China |
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Abstract Pannexin-1 (Panx1) forms nonselective large channel in cell plasma membrane and has been shown to be associated with NLRP3 inflammasome activation, ATP release and phagocytes recruitment. In the current study, by manipulation of Panx1 expression in human myeloid cells and application of Panx1 defi cient mice, we failed to fi nd a correlation between Panx1 and NLRP3 inflammasome activation, although an interaction between these two proteins was evident. However, in thioglycollate induced peritonitis, Panx1 defi cient mice showed much more phagocytes infiltration. Further analyses showed that mice defi cient for Panx1 exhibited enlarged F4/80lowGr1-Ly6C-cell population in the peritonea. Our study thus reveals an important role for Panx1 in regulation of peritoneal cell population and peritonitis development.
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| Keywords
innate Immunity
inflammasome
NLRP3
Pannexin-1
peritonitis
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Corresponding Author(s):
Meng Guangxun,Email:gxmeng@sibs.ac.cn
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Issue Date: 01 April 2013
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