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Human pathogenic fungus Trichophytonschoenleinii activates the NLRP3 inflammasome |
Hua Li1,2, Shuxian Wu2, Liming Mao2, Guowei Lei1,2, Liping Zhang3, Ailing Lu2, Liguo An1, Guiwen Yang1, Paride Abliz3( ), Guangxun Meng2() |
| 1. College of Life Science, Shandong Normal University, Jinan 250014, China; 2. Unit of Innate Immunity, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China; 3. Department of Dermatology, The First Hospital of Xinjiang Medical University, Urumqi 830054, China |
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Abstract The fungus Trichophyton schoenleinii (T. schoenleinii) is the causative agent of Trichophytosis and Tinea favosa of the scalp in certain regions of Eurasia and Africa. Human innate immune system plays an important role in combating with various pathogens including fungi. The inflammasome is one of the most critical arms of host innate immunity, which is a protein complex controlling maturation of IL-1β. To clarify whether T. schoenleinii is able to activate the inflammasome, we analyzed human monocytic cell line THP-1 for IL-1β production upon infection with T. schoenleinii strain isolated from Tinea favosa patients, and rapid IL-1β secretion from THP-1 cells was observed. Moreover, applying competitive inhibitors and gene specific silencing with shRNA, we found that T. schoenleinii induced IL-1β secretion, ASC pyroptosome formation as well as caspase-1 activation were all dependent on NLRP3. Cathepsin B activity, ROS production and K+ efflux were required for the inflammasome activation by T. schoenleinii. Our data thus reveal that the NLRP3 inflammasome plays an important role in host defense against T. schoenleinii, and suggest that manipulating NLRP3 signaling can be a novel approach for control of diseases caused by T. schoenleinii infection.
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| Keywords
innate immunity
inflammasome
NLRP3
Trichophyton schoenleinii
infection
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Corresponding Author(s):
Abliz Paride,Email:palidaa@yahoo.com.cn; Meng Guangxun,Email:gxmeng@sibs.ac.cn
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Issue Date: 01 July 2013
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