<i>In vitro</i> culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration

doi:10.1007/s13238-015-0180-2

Protein Cell

2015, Vol. 6

Issue (8) : 562-574 https://doi.org/10.1007/s13238-015-0180-2

REVIEW

In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration

Chenxia Hu,Lanjuan Li(

)

Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou 310006, China

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Abstract

Various liver diseases result in terminal hepatic failure, and liver transplantation, cell transplantation and artificial liver support systems are emerging as effective therapies for severe hepatic disease. However, all of these treatments are limited by organ or cell resources, so developing a sufficient number of functional hepatocytes for liver regeneration is a priority. Liver regeneration is a complex process regulated by growth factors (GFs), cytokines, transcription factors (TFs), hormones, oxidative stress products, metabolic networks, and microRNA. It is well-known that the function of isolated primary hepatocytes is hard to maintain; when cultured in vitro, these cells readily undergo dedifferentiation, causing them to lose hepatocyte function. For this reason, most studies focus on inducing stem cells, such as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), hepatic progenitor cells (HPCs), and mesenchymal stem cells (MSCs), to differentiate into hepatocyte-like cells (HLCs) in vitro. In this review, we mainly focus on the nature of the liver regeneration process and discuss how to maintain and enhance in vitro hepatic functionof isolated primary hepatocytes or stem cell-derived HLCs for liver regeneration. In this way, hepatocytes or HLCs may be applied for clinical use for the treatment of terminal liver diseases and may prolong the survival time of patients in the near future.

Keywords liver regeneration primary hepatocyte stem cell hepatocyte-like cell in vitro culture

Corresponding Author(s): Lanjuan Li

Issue Date: 05 August 2015

Cite this article:

Chenxia Hu,Lanjuan Li. In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration[J]. Protein Cell, 2015, 6(8): 562-574.

URL:

https://academic.hep.com.cn/pac/EN/10.1007/s13238-015-0180-2
https://academic.hep.com.cn/pac/EN/Y2015/V6/I8/562

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