Transcriptome resources and genome-wide marker development for Japanese larch (<i>Larix kaempferi</i>)

doi:10.15302/J-FASE-2014010

Front. Agr. Sci. Eng.

2014, Vol. 1

Issue (1) : 77-84 https://doi.org/10.15302/J-FASE-2014010

RESEARCH ARTICLE

Transcriptome resources and genome-wide marker development for Japanese larch (Larix kaempferi)

Wanfeng LI¹,Suying HAN²,Liwang QI¹,Shougong ZHANG^1,^*(

)

1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

Download: PDF(621 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract

While the differential responses of trees to changes in climatic and environmental conditions have been demonstrated as they age, the underlying mechanisms and age control of tree growth and development are complex and poorly understood particularly at a molecular level. In this paper, we present a transcriptome analysis of Larix kaempferi, a deciduous conifer that is widely-grown in the northern hemisphere and of significant ecological and economic value. Using high-throughput RNA sequencing, we obtained about 26 million reads from the stems of 1-, 2-, 5-, 10-, 25- and 50-year-old L. kaempferi trees. Combining these with the published Roche 454 sequencing reads and the expressed sequence tags (both mainly from Larix embryogenic cell cultures), we assembled 26670549 reads into 146786 transcripts, of which we annotated 79182 to support investigations of the molecular basis of tree aging and adaption, somatic embryogenesis and wood formation. Using these sequences we also identified many single-nucleotide polymorphisms, simple sequence repeats, and insertion and deletion markers to assist breeding and genetic diversity studies of Larix.

Keywords Larix transcriptome age wood formation somatic embryogenesis molecular marker

Corresponding Author(s): Shougong ZHANG

Issue Date: 22 May 2014

Cite this article:

Wanfeng LI,Suying HAN,Liwang QI, et al. Transcriptome resources and genome-wide marker development for Japanese larch (Larix kaempferi)[J]. Front. Agr. Sci. Eng. , 2014, 1(1): 77-84.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2014010
https://academic.hep.com.cn/fase/EN/Y2014/V1/I1/77

Tab.1 Summary of transcriptome assembly

Fig.1 Representation of Gene Ontology (GO) classification in terms of ‘biologic processes’

Fig.2 Phylogenetic tree analysis from the orthologous data set across A. thaliana, L. kaempferi, P. abies, P. trichocarpa and V. vinifera

Tab.2 Molecular markers identified from L. kaempferi

Fig.3 Summary analysis of single-nucleotide polymorphisms (SNPs) and single-nucleotide polymorphisms (SSRs). (a) Distribution of minor allele frequencies of SNPs identified in L. kaempferi. The x-axis represents the SNP sequence-derived minor allele frequency, while the y-axis represents the number of SNPs with a given minor allele frequency; (b) distribution of SNPs per transcript. The x-axis represents the number of SNPs per transcript, while the y-axis represents the number of transcripts with a given number of SNPs; (c) frequency of SSRs. The x-axis represents the SSR types and the y-axis represents the percentage of a given SSR type

1	Rossi S, Deslauriers A, Anfodillo T, Carrer M. Age-dependent xylogenesis in timberline conifers. New Phytologist, 2008, 177(1): 199–20817944824 https://doi.org/10.1111/j.1469-8137.2007.02235.x
2	Rossi S, Deslauriers A, Gri?ar J, Seo J W, Rathgeber C B K, Anfodillo T, Morin H, Levanic T, Oven P, Jalkanen R. Critical temperatures for xylogenesis in conifers of cold climates. Global Ecology and Biogeography, 2008, 17(6): 696–707 https://doi.org/10.1111/j.1466-8238.2008.00417.x
3	Begum S, Nakaba S, Oribe Y, Kubo T, Funada R. Cambial sensitivity to rising temperatures by natural condition and artificial heating from late winter to early spring in the evergreen conifer Cryptomeria japonica. Trees-Structure and Function, 2010, 24(1): 43–52 https://doi.org/10.1007/s00468-009-0377-1
4	Li X, Liang E, Gri?ar J, Prislan P, Rossi S, ?ufar K. Age dependence of xylogenesis and its climatic sensitivity in Smith fir on the south-eastern Tibetan Plateau. Tree Physiology, 2013, 33(1): 48–56 https://doi.org/10.1093/treephys/tps113 pmid: 23185065
5	Li W F, Ding Q, Chen J J, Cui K M, He X Q. Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr. Journal of Experimental Botany, 2009, 60(9): 2621–2630 https://doi.org/10.1093/jxb/erp108 pmid: 19414499
6	Little C H A, Bonga J M. Rest in cambium of Abies balsamea. Canadian Journal of Botany, 1974, 52(7): 1723–1730 https://doi.org/10.1139/b74-224
7	Mwange K N, Wang X W, Cui K M. Mechanism of dormancy in the buds and cambium of Eucommia ulmoides. Acta Botanica Sinica, 2003, 45(6): 698–704
8	Mwange K N, Hou H W, Wang Y Q, He X Q, Cui K M. Opposite patterns in the annual distribution and time-course of endogenous abscisic acid and indole-3-acetic acid in relation to the periodicity of cambial activity in Eucommia ulmoides Oliv. Journal of Experimental Botany, 2005, 56(413): 1017–1028 https://doi.org/10.1093/jxb/eri095 pmid: 15710633
9	Sundberg B, Little C H A. Tracheid production in response to changes in the internal level of indole-3-acetic Acid in 1-year-old shoots of scots pine. Plant Physiology, 1990, 94(4): 1721–1727 https://doi.org/10.1104/pp.94.4.1721 pmid: 16667908
10	Baba K, Karlberg A, Schmidt J, Schrader J, Hvidsten T R, Bako L, Bhalerao R P. Activity-dormancy transition in the cambial meristem involves stage-specific modulation of auxin response in hybrid aspen. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(8): 3418–3423 https://doi.org/10.1073/pnas.1011506108 pmid: 21289280
11	Nilsson J, Karlberg A, Antti H, Lopez-Vernaza M, Mellerowicz E, Perrot-Rechenmann C, Sandberg G, Bhalerao R P. Dissecting the molecular basis of the regulation of wood formation by auxin in hybrid aspen. Plant Cell, 2008, 20(4): 843–855 https://doi.org/10.1105/tpc.107.055798 pmid: 18424614
12	Savidge R A, Wareing P F. A tracheid-differentiation factor from pine needles. Planta, 1981, 153(5): 395–404 https://doi.org/10.1007/BF00394977 pmid: 24275808
13	Little C H A, Sundberg B. Tracheid production in response to indole-3-acetic-acid varies with internode age in Pinus sylvestris stems. Trees-Structure and Function, 1991, 5(2): 101–106 https://doi.org/10.1007/BF00227492
14	Savidge R A. The role of plant hormones in higher plant cellular differentiation. II. Experiments with the vascular cambium, and sclereid and tracheid differentiation in the pine, Pinus contorta. Histochemical Journal, 1983, 15(5): 447–466 https://doi.org/10.1007/BF01002699 pmid: 6223902
15	Alvarez C, Valledor L. R. H, Sanchez-Olate M, Ríos D. Variation in gene expression profile with aging of Pinus radiata D. Don. BMC Proceedings, 2011, 5 (Suppl 7): P62 https://doi.org/10.1186/1753-6561-5-S7-P62
16	Busov V B, Johannes E, Whetten R W, Sederoff R R, Spiker S L, Lanz-Garcia C, Goldfarb B. An auxin-inducible gene from loblolly pine (Pinus taeda L.) is differentially expressed in mature and juvenile-phase shoots and encodes a putative transmembrane protein. Planta, 2004, 218(6): 916–927 https://doi.org/10.1007/s00425-003-1175-4 pmid: 14722770
17	Carlsbecker A, Tandre K, Johanson U, Englund M, Engstr?m P. The MADS-box gene DAL1 is a potential mediator of the juvenile-to-adult transition in Norway spruce (Picea abies). Plant Journal, 2004, 40(4): 546–557 https://doi.org/10.1111/j.1365-313X.2004.02226.x pmid: 15500470
18	Diego L B, Berdasco M, Fraga M F, Ca?al M J, Rodríguez R, Castresana C. A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing. Journal of Experimental Botany, 2004, 55(402): 1597–1599 https://doi.org/10.1093/jxb/erh171 pmid: 15208342
19	Fernández-Oca?a A, Carmen García-López M, Jiménez-Ruiz J, Saniger L, Macías D, Navarro F, Oya R, Belaj A, de la Rosa R, Corpas F J, Bautista Barroso J, Luque F. Identification of a gene involved in the juvenile-to-adult transition (JAT) in cultivated olive trees. Tree Genetics & Genomes, 2010, 6(6): 891–903 https://doi.org/10.1007/s11295-010-0299-5
20	Hutchison K W, Sherman C D, Weber J, Smith S S, Singer P B, Greenwood M S. Maturation in larch: II. effects of age on photosynthesis and gene expression in developing foliage. Plant Physiology, 1990, 94(3): 1308–1315 https://doi.org/10.1104/pp.94.3.1308 pmid: 16667834
21	Li X, Wu H X, Southerton S G. Seasonal reorganization of the xylem transcriptome at different tree ages reveals novel insights into wood formation in Pinus radiata. New Phytologist, 2010, 187(3): 764–776 https://doi.org/10.1111/j.1469-8137.2010.03333.x pmid: 20561208
22	Li X, Wu H X, Southerton S G. Transcriptome profiling of wood maturation in Pinus radiata identifies differentially expressed genes with implications in juvenile and mature wood variation. Gene, 2011, 487(1): 62–71 https://doi.org/10.1016/j.gene.2011.07.028 pmid: 21839815
23	Wang J W, Park M Y, Wang L J, Koo Y, Chen X Y, Weigel D, Poethig R S. miRNA control of vegetative phase change in trees. PLOS Genetics, 2011, 7(2): e1002012 https://doi.org/10.1371/journal.pgen.1002012 pmid: 21383862
24	Hsu C Y, Liu Y, Luthe D S, Yuceer C. Poplar FT2 shortens the juvenile phase and promotes seasonal flowering. Plant Cell, 2006, 18(8): 1846–1861 https://doi.org/10.1105/tpc.106.041038 pmid: 16844908
25	Hsu C Y, Adams J P, Kim H, No K, Ma C, Strauss S H, Drnevich J, Vandervelde L, Ellis J D, Rice B M, Wickett N, Gunter L E, Tuskan G A, Brunner A M, Page G P, Barakat A, Carlson J E, DePamphilis C W, Luthe D S, Yuceer C. FLOWERING LOCUS T duplication coordinates reproductive and vegetative growth in perennial poplar. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(26): 10756–10761 https://doi.org/10.1073/pnas.1104713108 pmid: 21653885
26	B?hlenius H, Huang T, Charbonnel-Campaa L, Brunner A M, Jansson S, Strauss S H, Nilsson O. CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees. Science, 2006, 312(5776): 1040–1043 https://doi.org/10.1126/science.1126038 pmid: 16675663
27	Li S G, Li W F, Han S Y, Yang W H, Qi L W. Stage-specific regulation of four HD-ZIP III transcription factors during polar pattern formation in Larix leptolepis somatic embryos. Gene, 2013, 522(2): 177–183 https://doi.org/10.1016/j.gene.2013.03.117 pmid: 23566830
28	Li W F, Zhang S G, Han S Y, Wu T, Zhang J H, Qi L W. Regulation of LaMYB33 by miR159 during maintenance of embryogenic potential and somatic embryo maturation in Larix kaempferi (Lamb.) Carr. Plant Cell, Tissue and Organ Culture, 2013, 113(1): 131–136 https://doi.org/10.1007/s11240-012-0233-7
29	Zhang J, Zhang S, Han S, Li X, Tong Z, Qi L. Deciphering small noncoding RNAs during the transition from dormant embryo to germinated embryo in Larches (Larix leptolepis). PLoS ONE, 2013, 8(12): e81452 https://doi.org/10.1371/journal.pone.0081452 pmid: 24339932
30	Zhang J, Zhang S, Han S, Wu T, Li X, Li W, Qi L. Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis. Planta, 2012, 236(2): 647–657 https://doi.org/10.1007/s00425-012-1643-9 pmid: 22526500
31	Zhang L F, Li W F, Han S Y, Yang W H, Qi L W. cDNA cloning, genomic organization and expression analysis during somatic embryogenesis of the translationally controlled tumor protein (TCTP) gene from Japanese larch (Larix leptolepis). Gene, 2013, 529(1): 150–158 https://doi.org/10.1016/j.gene.2013.07.076 pmid: 23933269
32	Zhang S, Zhou J, Han S, Yang W, Li W, Wei H, Li X, Qi L. Four abiotic stress-induced miRNA families differentially regulated in the embryogenic and non-embryogenic callus tissues of Larix leptolepis. Biochemical and Biophysical Research Communications, 2010, 398(3): 355–360 https://doi.org/10.1016/j.bbrc.2010.06.056 pmid: 20599742
33	Zhang S G, Han S Y, Yang W H, Wei H L, Zhang M, Qi L W. Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis. Plant Cell, Tissue and Organ Culture, 2010, 100(1): 21–29 https://doi.org/10.1007/s11240-009-9612-0
34	Li W F, Zhang S G, Han S Y, Wu T, Zhang J H, Qi L W. The post-transcriptional regulation of LaSCL6 by miR171 during maintenance of embryogenic potential in Larix kaempferi (Lamb.). Carr. Tree Genetics & Genomes, 2014, 10(1): 223–229 https://doi.org/10.1007/s11295-013-0668-y
35	Zhang J H, Zhang S G, Li S G, Han S Y, Li W F, Li X M, Qi L W. Regulation of synchronism by abscisic-acid-responsive small noncoding RNAs during somatic embryogenesis in larch (Larix leptolepis). Plant Cell, Tissue and Organ Culture, 2014, 116(3): 361–370 https://doi.org/10.1007/s11240-013-0412-1
36	Zhang Y, Zhang S, Han S, Li X, Qi L. Transcriptome profiling and in silico analysis of somatic embryos in Japanese larch (Larix leptolepis). Plant Cell Reports, 2012, 31(9): 1637–1657 https://doi.org/10.1007/s00299-012-1277-1 pmid: 22622308
37	Men L, Yan S, Liu G. De novo characterization of Larix gmelinii (Rupr.) Rupr. transcriptome and analysis of its gene expression induced by jasmonates. BMC Genomics, 2013, 14(1): 548 https://doi.org/10.1186/1471-2164-14-548 pmid: 23941306
38	Mackay J, Dean J F, Plomion C, Peterson D G, Cánovas F M, Pavy N, Ingvarsson P K, Savolainen O, Guevara M á, Fluch S, Vinceti B, Abarca D, Díaz-Sala C, Cervera M T. Towards decoding the conifer giga-genome. Plant Molecular Biology, 2012, 80(6): 555–569 https://doi.org/10.1007/s11103-012-9961-7 pmid: 22960864
39	Grabherr M G, Haas B J, Yassour M, Levin J Z, Thompson D A, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren B W, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 2011, 29(7): 644–652 https://doi.org/10.1038/nbt.1883 pmid: 21572440
40	Zhang L, Qi L W, Han S Y. [Differentially expressed genes during Larix somatic embryomaturation and the expression profile of partial genes. Hereditas, 2009, 31(5): 540–545 (in Chinese) https://doi.org/10.3724/SP.J.1005.2009.00540 pmid: 19586850
41	Zhang L, Qi L, Han S. Construction and analysis of differentially expressed cDNA library of larch somatic embryo at the stage of proembryogenic mass. Molecular Plant Breeding, 2008, 6(4): 675–682 (in Chinese) https://doi.org/10.3969/j.issn.1672-416X.2008.04.009
42	Huang X, Madan A. CAP3: A DNA sequence assembly program. Genome Research, 1999, 9(9): 868–877 https://doi.org/10.1101/gr.9.9.868 pmid: 10508846
43	Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. Basic local alignment search tool. Journal of Molecular Biology, 1990, 215(3): 403–410 https://doi.org/10.1016/S0022-2836(05)80360-2 pmid: 2231712
44	Guindon S, Dufayard J F, Lefort V, Anisimova M, Hordijk W, Gascuel O. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 2010, 59(3): 307–321 https://doi.org/10.1093/sysbio/syq010 pmid: 20525638
45	Li H, Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics, 2009, 25(14): 1754–1760 https://doi.org/10.1093/bioinformatics/btp324 pmid: 19451168
46	Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R. The Sequence Alignment/Map format and SAMtools. Bioinformatics, 2009, 25(16): 2078–2079 https://doi.org/10.1093/bioinformatics/btp352 pmid: 19505943
47	Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth B C, Remm M, Rozen S G. Primer3-new capabilities and interfaces. Nucleic Acids Research, 2012, 40(15): e115 https://doi.org/10.1093/nar/gks596 pmid: 22730293
48	Carrer M, Urbinati C. Age-dependent tree-ring growth responses to climate in Larix decidua and Pinus cembra. Ecology, 2004, 85(3): 730–740 https://doi.org/10.1890/02-0478
49	Tian Z H, Dong J, Wang X W, Huang G R. Silviculture of Larix kaempferi. 1995, Beijing: Beijing Agriculture University Press94–105. (in Chinese)
50	Sorce C, Giovannelli A, Sebastiani L, Anfodillo T. Hormonal signals involved in the regulation of cambial activity, xylogenesis and vessel patterning in trees. Plant Cell Reports, 2013, 32(6): 885–898 https://doi.org/10.1007/s00299-013-1431-4 pmid: 23553557
51	Sehr E M, Agusti J, Lehner R, Farmer E E, Schwarz M, Greb T. Analysis of secondary growth in the Arabidopsis shoot reveals a positive role of jasmonate signalling in cambium formation. Plant Journal, 2010, 63(5): 811–822 https://doi.org/10.1111/j.1365-313X.2010.04283.x pmid: 20579310
52	Cairney J, Pullman G S. The cellular and molecular biology of conifer embryogenesis. New Phytologist, 2007, 176(3): 511–536 https://doi.org/10.1111/j.1469-8137.2007.02239.x pmid: 17953539
53	Quiroz-Figueroa F R, Rojas-Herrera R, Galaz-Avalos R M, Loyola-Vargas V M. Embryo production through somatic embryogenesis can be used to study cell differentiation in plants. Plant Cell, Tissue and Organ Culture, 2006, 86(3): 285–301 https://doi.org/10.1007/s11240-006-9139-6
54	Zimmerman J L. Somatic embryogenesis: a model for early development in higher plants. Plant Cell, 1993, 5(10): 1411–1423 https://doi.org/10.1105/tpc.5.10.1411 pmid: 12271037
55	Gutmann M, vonAderkas P, Label P, Lelu M A. Effects of abscisic acid on somatic embryo maturation of hybrid larch. Journal of Experimental Botany, 1996, 47(12): 1905–1917 https://doi.org/10.1093/jxb/47.12.1905
56	Rai M K, Shekhawat N S, Harish, Gupta A K, Phulwaria M, Ram K, Jaiswal U. Harish, Gupta A K, Phulwaria M, Ram K, Jaiswal U. The role of abscisic acid in plant tissue culture: a review of recent progress. Plant Cell, Tissue and Organ Culture, 2011, 106(2): 179–190 https://doi.org/10.1007/s11240-011-9923-9
57	Khasa D P, Jaramillo-Correa J P, Jaquish B, Bousquet J. Contrasting microsatellite variation between subalpine and western larch, two closely related species with different distribution patterns. Molecular Ecology, 2006, 15(13): 3907–3918 https://doi.org/10.1111/j.1365-294X.2006.03066.x pmid: 17054492
58	Pluess A R. Pursuing glacier retreat: genetic structure of a rapidly expanding Larix decidua population. Molecular Ecology, 2011, 20(3): 473–485 https://doi.org/10.1111/j.1365-294X.2010.04972.x pmid: 21199030
59	Oreshkova N V, Belokon M M, Jamiyansuren S. Genetic diversity, population structure, and differentiation of Siberian larch, Gmelin larch and Cajander larch on SSR-markers data. Russian Journal of Genetics, 2013, 49(2): 178–186 https://doi.org/10.1134/S1022795412120095 pmid: 23668086
60	Kozyrenko M M, Artyukova E V, Reunova G D, Levina E A, Zhuravlev Y N. Genetic diversity and relationships among Siberian and Far Eastern larches inferred from RAPD analysis. Russian Journal of Genetics, 2004, 40(4): 401–409 https://doi.org/10.1023/B:RUGE.0000024978.25458.f7
61	Yu X M, Zhou Q, Qian Z Q, Li S, Zhao G F. Analysis of genetic diversity and population differentiation of Larix potaninii var.chinensis using microsatellite DNA. Biochemical Genetics, 2006, 44(11–12): 483–493 https://doi.org/10.1007/s10528-006-9049-7 pmid: 17103046
62	Funda T, Chen C, Liewlaksaneeyanawin C, Kenawy A M A, El-Kassaby Y A.C.. Liewlaksaneeyanawin C, Kenawy A M A, El-Kassaby Y A.. Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population. Annals of Forest Science, 2008, 65(7): 705 https://doi.org/10.1051/forest:2008055
63	Acheré V, Faivre Rampant P, Paques L E, Prat D. Chloroplast and mitochondrial molecular tests identify European × Japanese larch hybrids. Theoretical and Applied Genetics, 2004, 108(8): 1643–1649 https://doi.org/10.1007/s00122-004-1595-y pmid: 14991107
64	Yang X, Sun X, Zhang S, Xie Y, Han H. Development of EST-SSR markers and genetic diversity analysis of the second cycle elite population in Larix kaempferi. Scientia Silvae Sinicae, 2011, 47(11): 52–58 (in Chinese) https://doi.org/10.11707/j.1001-7488.20111109
65	Liu C, Zhang L P, Wang C G, Song W Q, Chen C B. Development and Characterization of EST-SSR Molecular Markers in Larix kaempferi.Forest reseach, 2013, 26(S1): 60–68. (in Chinese)
66	Wagner S, Gerber S, Petit R J. Two highly informative dinucleotide SSR multiplexes for the conifer Larix decidua (European larch). Molecular ecology resources, 2012, 12(4): 717–25 https://doi.org/10.1111/j.1755-0998.2012.03139.x

[1]	Andreas BUERKERT, Eva SCHLECHT. Toward sustainable intensification of agriculture in sub-Saharan Africa[J]. Front. Agr. Sci. Eng. , 2020, 7(4): 401-405.
[2]	Enli WANG, Di HE, Zhigan ZHAO, Chris J. SMITH, Ben C. T. MACDONALD. Using a systems modeling approach to improve soil management and soil quality[J]. Front. Agr. Sci. Eng. , 2020, 7(3): 289-295.
[3]	Yakov KUZYAKOV, Anna GUNINA, Kazem ZAMANIAN, Jing TIAN, Yu LUO, Xingliang XU, Anna YUDINA, Humberto APONTE, Hattan ALHARBI, Lilit OVSEPYAN, Irina KURGANOVA, Tida GE, Thomas GUILLAUME. New approaches for evaluation of soil health, sensitivity and resistance to degradation[J]. Front. Agr. Sci. Eng. , 2020, 7(3): 282-288.
[4]	Giulia BONGIORNO. Novel soil quality indicators for the evaluation of agricultural management practices: a biological perspective[J]. Front. Agr. Sci. Eng. , 2020, 7(3): 257-274.
[5]	Nicolas MUNIER-JOLAIN, Martin LECHENET. Methodological considerations for redesigning sustainable cropping systems: the value of data-mining large and detailed farm data sets at the cropping system level[J]. Front. Agr. Sci. Eng. , 2020, 7(1): 21-27.
[6]	Zhenling CUI, Zhengxia DOU, Hao YING, Fusuo ZHANG. Producing more with less: reducing environmental impacts through an integrated soil-crop system management approach[J]. Front. Agr. Sci. Eng. , 2020, 7(1): 14-20.
[7]	Mahmood ul HASSAN, Xin WEN, Jiuliang XU, Jiahui ZHONG, Xuexian LI. Development and challenges of green food in China[J]. Front. Agr. Sci. Eng. , 2020, 7(1): 56-66.
[8]	David R. CHADWICK, John R. WILLIAMS, Yuelai LU, Lin MA, Zhaohai BAI, Yong HOU, Xinping CHEN, Thomas H. MISSELBROOK. Strategies to reduce nutrient pollution from manure management in China[J]. Front. Agr. Sci. Eng. , 2020, 7(1): 45-55.
[9]	Rui WANG, Weiming SHI, Yilin LI. Phosphorus supply and management in vegetable production systems in China[J]. Front. Agr. Sci. Eng. , 2019, 6(4): 348-356.
[10]	Jianbo SHEN, Liyang WANG, Xiaoqiang JIAO, Fanlei MENG, Lin ZHANG, Gu FENG, Junling ZHANG, Lixing YUAN, Lin MA, Yong HOU, Tao ZHANG, Weifeng ZHANG, Guohua LI, Kai ZHANG, Fusuo ZHANG. Innovations of phosphorus sustainability: implications for the whole chain[J]. Front. Agr. Sci. Eng. , 2019, 6(4): 321-331.
[11]	Qing XUE, Xinyue HE, Saskia D. SACHS, Gero C. BECKER, Tao ZHANG, Andrea KRUSE. The current phosphate recycling situation in China and Germany: a comparative review[J]. Front. Agr. Sci. Eng. , 2019, 6(4): 403-418.
[12]	Wujun MA, Zitong YU, Maoyun SHE, Yun ZHAO, Shahidul ISLAM. Wheat gluten protein and its impacts on wheat processing quality[J]. Front. Agr. Sci. Eng. , 2019, 6(3): 279-287.
[13]	Rudi APPELS. Wheat research and breeding in the new era of a high-quality reference genome[J]. Front. Agr. Sci. Eng. , 2019, 6(3): 225-232.
[14]	Meng DUAN, Jin XIE, Xiaomin MAO. Modeling water and heat transfer in soil-plant-atmosphere continuum applied to maize growth under plastic film mulching[J]. Front. Agr. Sci. Eng. , 2019, 6(2): 144-161.
[15]	Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU. Cryopreservation of farm animal gametes and embryos: recent updates and progress[J]. Front. Agr. Sci. Eng. , 2019, 6(1): 42-53.

Viewed

Full text

Abstract

Cited

Shared

Discussed