Expression profiles of genes and enzymes related to ascorbic acid metabolism in fruits of <i>Ziziphus jujuba</i> Mill. ‘Jinsixiaozao’

doi:10.15302/J-FASE-2016096

Front. Agr. Sci. Eng.

2016, Vol. 3

Issue (2) : 131-136 https://doi.org/10.15302/J-FASE-2016096

RESEARCH ARTICLE

Expression profiles of genes and enzymes related to ascorbic acid metabolism in fruits of Ziziphus jujuba Mill. ‘Jinsixiaozao’

Yingying CHEN¹,Zhihui ZHAO¹,Jin ZHAO^2,^*(

),Mengjun LIU^1,^*(

)

¹. Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China
². College of Life Science, Agricultural University of Hebei, Baoding 071000, China

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Abstract

The fruit of Chinese jujube (Ziziphus jujuba) possesses extremely high concentrations of ascorbic acid (AsA). The accumulation of AsA, the expression patterns of the nine genes related to AsA metabolism as well as the activities of five enzymes involved in AsA synthesis, oxidation and recycling were investigated during fruit development in Z. jujuba Mill. ‘Jinsixiaozao’. The results showed that the high level of AsA accumulation in jujube fruit is due to a contribution from both AsA biosynthesis and AsA recycling. It is suggested that L-galactono-1,4-lactone dehydrogenase, ascorbate peroxidase and monodehydro-ascorbate reductase are the crucial genes/enzymes of jujube AsA synthesis, oxidization and recycling, respectively. These results provide useful new insights into the regulatory mechanisms of AsA accumulation in Chinese jujube.

Keywords Chinese jujube ascorbic acid metabolism gene enzyme

Corresponding Author(s): Jin ZHAO,Mengjun LIU

Just Accepted Date: 25 April 2016 Online First Date: 10 May 2016 Issue Date: 05 July 2016

Cite this article:

Yingying CHEN,Zhihui ZHAO,Jin ZHAO, et al. Expression profiles of genes and enzymes related to ascorbic acid metabolism in fruits of Ziziphus jujuba Mill. ‘Jinsixiaozao’[J]. Front. Agr. Sci. Eng. , 2016, 3(2): 131-136.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2016096
https://academic.hep.com.cn/fase/EN/Y2016/V3/I2/131

Fig.1 Changes of fruit diameter and weight during fruit development (a) and ascorbic acid concentration of fruit during fruit development (b). a’, b’, c’ and d’ mean the significant level at 5%. I, young fruit stage; II, pre-white ripening stage; III, white ripening stage; IV, half-red ripening stage; V, full-red ripening stage.

Fig.2 Relative expressions of genes involved in AsA biosynthesis and recycling in jujube fruit. Enzymes studied were: GDP-D-mannose pyrophosphorylase (GMP), GDP-mannose 3′,5′-epimerase (GME), GDP-L-galactose phosphorylase (GGP); L-galactose-1-P phosphatase (GPP), L-galactose dehydrogenase (GLDH), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), ascorbate peroxidase (APX), ascorbate oxidase (AO). I, young fruit stage; II, pre-white ripening stage; III, white ripening stage; IV, half-red ripening stage; V, full-red ripening stage.

Tab.1 Changes in the activity of five selected enzymes during jujube fruit development (U·g^-1)

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