Optimization and modeling of biohydrogen production by mixed bacterial cultures from raw cassava starch

doi:10.1007/s11705-017-1617-3

Front. Chem. Sci. Eng.

2017, Vol. 11

Issue (1) : 100-106 https://doi.org/10.1007/s11705-017-1617-3

RESEARCH ARTICLE

Optimization and modeling of biohydrogen production by mixed bacterial cultures from raw cassava starch

Shaojie Wang,Zhihong Ma,Ting Zhang,Meidan Bao,Haijia Su(

)

Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China

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Abstract

The production of bio-hydrogen from raw cassava starch via a mixed-culture dark fermentation process was investigated. The production yield of H₂ was optimized by adjusting the substrate concentration and the microorganism mixture ratio. A maximum H₂ yield of 1.72 mol H₂/mol glucose was obtained with a cassava starch concentration of 10 g/L to give a 90% utilization rate. The kinetics of the substrate utilization and of the generation of both hydrogen and volatile fatty acids were also investigated. The substrate utilization follows pseudo first order reaction kinetics, whereas the production of both H₂ and the VFAs correlate with the Gompertz equation. These results show that cassava is a good candidate for the production of biohydrogen.

Keywords cassava biohydrogen mixed cultures kinetics

Corresponding Author(s): Haijia Su

Just Accepted Date: 28 December 2016 Online First Date: 13 February 2017 Issue Date: 17 March 2017

Cite this article:

Shaojie Wang,Zhihong Ma,Ting Zhang, et al. Optimization and modeling of biohydrogen production by mixed bacterial cultures from raw cassava starch[J]. Front. Chem. Sci. Eng., 2017, 11(1): 100-106.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-017-1617-3
https://academic.hep.com.cn/fcse/EN/Y2017/V11/I1/100

Fig.1 Cumulative H₂ production and pH with time for different substrate concentrations for A1:B1=1:1. Filled symbols are cumulative H₂ production and hollow symbols are pH

Tab.1 Hydrogen yields and concentration for different substrate concentrations

Fig.2 Types of VFAs produced with different substrate concentrations (A1:B1=1:1)

Fig.3 Cumulative H₂ production with time with different mixture ratios

Fig.4 First order kinetic fit of substrate utilization at different mixture ratios

Tab.2 First order k values and correlation coefficients

Fig.5 Gompertz fitted curve for the cumulative H₂ production for different mixture ratios

Fig.6 Gompertz fit curve of VFAs generation at different mixture ratios.

(a) A1/B1=1/0.5; (b) A1/B1=1/1; (c) A1/B1=1/2; (d) A1/B1=1/3

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