Loss-of-function of so<i>x3</i> causes follicle development retardation and reduces fecundity in zebrafish

doi:10.1007/s13238-018-0603-y

Protein Cell

2019, Vol. 10

Issue (5) : 347-364 https://doi.org/10.1007/s13238-018-0603-y

RESEARCH ARTICLE

Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish

Qiang Hong, Cong Li, Ruhong Ying, Heming Lin, Jingqiu Li, Yu Zhao, Hanhua Cheng(

), Rongjia Zhou(

)

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 430072, China

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Abstract

Folliculogenesis is essential for production of female gametes in vertebrates. However, the molecular mechanisms underlying follicle development, particularly apoptosis regulation in ovary, remain elusive. Here, we generated sox3 knockout zebrafish lines using CRISPR/Cas9. sox3 knockout led to follicle development retardation and a reduced fecundity in females. Comparative analysis of transcriptome between sox3^−/− and wild-type ovaries revealed that Sox3 was involved in pathways of ovarian steroidogenesis and apoptosis. Knockout of sox3 promoted follicle apoptosis and obvious apoptosis signals were detected in somatic cells of stages III and IV follicles of sox3^−/− ovaries. Moreover, Sox3 can bind to and activate the promoter of cyp19a1a. Up-regulation of Cyp19a1a expression promoted 17β-estradiol synthesis, which inhibited apoptosis in follicle development. Thus, Sox3 functions as a regulator of Cyp19a1a expression, via 17β-E2 linking apoptosis suppression, which is implicated in improving female fecundity.

Keywords Sox3 follicle development apoptosis Cyp19a1a zebrafish

Corresponding Author(s): Hanhua Cheng,Rongjia Zhou

Issue Date: 14 May 2019

Cite this article:

Qiang Hong,Cong Li,Ruhong Ying, et al. Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish[J]. Protein Cell, 2019, 10(5): 347-364.

URL:

https://academic.hep.com.cn/pac/EN/10.1007/s13238-018-0603-y
https://academic.hep.com.cn/pac/EN/Y2019/V10/I5/347

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