Apaf1 inhibition promotes cell recovery from apoptosis

doi:10.1007/s13238-015-0200-2

Protein Cell

2015, Vol. 6

Issue (11) : 833-843 https://doi.org/10.1007/s13238-015-0200-2

RESEARCH ARTICLE

Apaf1 inhibition promotes cell recovery from apoptosis

Anna Gortat¹,Mónica Sancho¹,Laura Mondragón¹,Àgel Messeguer²,Enrique Pérez-Payá^1,³,Mar Orzáez^1,^*(

)

1. Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, 46012 Valencia, Spain
2. Department of Chemical and Biomolecular Nanotechnology, Instituto Química Avanzada de Cataluña (CSIC), 08034 Barcelona, Spain
3. Instituto de Biomedicina de Valencia, IBV-CSIC, 46010 Valencia, Spain

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Abstract

The protein apoptotic protease activating factor 1 (Apaf1) is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apaf1, cells recover from doxorubicin- or hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apaf1 not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.

Keywords Apaf1 Apaf1 inhibitors apoptosis apoptosome autophagy cell recovery

Corresponding Author(s): Mar Orzáez

Issue Date: 04 November 2015

Cite this article:

Anna Gortat,Mónica Sancho,Laura Mondragón, et al. Apaf1 inhibition promotes cell recovery from apoptosis[J]. Protein Cell, 2015, 6(11): 833-843.

URL:

https://academic.hep.com.cn/pac/EN/10.1007/s13238-015-0200-2
https://academic.hep.com.cn/pac/EN/Y2015/V6/I11/833

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