Identification of genome regions and important variations associated with expression level of <i>MYH</i> genes and its correlation with meat quality traits in pigs

doi:10.15302/J-FASE-2015057

Front. Agr. Sci. Eng.

2015, Vol. 2

Issue (3) : 237-241 https://doi.org/10.15302/J-FASE-2015057

RESEARCH ARTICLE

Identification of genome regions and important variations associated with expression level of MYH genes and its correlation with meat quality traits in pigs

Ye HOU^1,²,Lu JING^1,²,Yunxia ZHAO^1,²,Sheng WANG^1,²,An LIU^1,²,Shuhong ZHAO^1,²,Xinyun LI^1,^2,^*(

)

1. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2. The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China

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Abstract

Meat quality is one of the most important economic traits in pig breeding. It has been reported that the composition of type II muscle fibers is correlated with meat quality in pigs. Type II muscle fibers contain three isoforms, IIa, IIb and IIx, which contain specific myofibrillar proteins MYH2, MYH4 and MYH1, respectively. In this study, the expression levels of MYH1, MYH2, and MYH4 genes in the Longissimus thoracis (LT) muscle were measured in 114 Yorkshire pigs. Further, the correlations between the expression level of MYH genes and the meat quality traits of intramuscular fat, drip loss, water-holding capacity and postmortem pH values were analyzed. The results showed that the expression level of MYH2 was positively correlated with the intramuscular fat (R= 0.20, P<0.05). The expression levels of MHY1 and MYH4 were negatively correlated with the pH at 24 h post mortem (pH_24h ; R= －0.28, P<0.01; R= －0.25, P<0.01, respectively). Besides, the 60K SNP chip was used for genotyping the individuals. Genome wide association analysis indicated that 15 SNPs were significantly associated with the expression levels of these three MYH genes. The results indicated the expression levels of MYH1, MYH2 and MYH4 genes could be useful markers for improvement of meat quality in pigs.

Keywords pig IMF pH MYH1 MYH2 MYH4

Corresponding Author(s): Xinyun LI

Just Accepted Date: 04 June 2015 Online First Date: 06 July 2015 Issue Date: 10 November 2015

Cite this article:

Ye HOU,Lu JING,Yunxia ZHAO, et al. Identification of genome regions and important variations associated with expression level of MYH genes and its correlation with meat quality traits in pigs[J]. Front. Agr. Sci. Eng. , 2015, 2(3): 237-241.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2015057
https://academic.hep.com.cn/fase/EN/Y2015/V2/I3/237

Tab.1 List of oligonucleotide primers of MYH genes

Fig.1 The expression level of MYH1, MYH2 and MYH4 genes in LT muscle in 114 Yorkshire pigs

Tab.2 Correlation coefficients for a matrix of expression levels of MYH genes and meat quality traits

Tab.3 SNPs and genes related to the expression levels of MYH genes

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