Synergistic degradation of pyrene and volatilization of arsenic by cocultures of bacteria and a fungus

doi:10.1007/s11783-012-0470-3

Front Envir Sci Eng

2013, Vol. 7

Issue (2) : 191-199 https://doi.org/10.1007/s11783-012-0470-3

RESEARCH ARTICLE

Synergistic degradation of pyrene and volatilization of arsenic by cocultures of bacteria and a fungus

Shuang LIU^1,2, Yanwei HOU³, Guoxin SUN¹(

)

1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2. School of Environment, Tsinghua University, Beijing 100084, China; 3. Department of Environmental Science and Technology, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China

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Abstract

The combination of two bacteria (Bacillus sp. PY1 and Sphingomonas sp. PY2) and a fungus (Fusarium sp. PY3), isolated from contaminated soils near a coking plant, were investigated with respect to their capability to degrade pyrene and volatilize arsenic. The results showed that all strains could use pyrene and arsenic as carbon and energy sources in a basal salts medium (BSM), with the combined potential to degrade pyrene and volatilize arsenic. Bacillus sp. PY1, Sphingomonas sp. PY2 and Fusarium sp. PY3 were isolated from the consortium and were shown to degrade pyrene and volatilize arsenic independently and in combination. Fungal-bacterial coculture has shown that the most effective removal of pyrene was 96.0% and volatilized arsenic was 84.1% after incubation in liquid medium after 9 days culture, while bioremediation ability was 87.2% in contaminated soil with 100 mg·kg^-1 pyrene. The highest level of arsenic volatilization amounted to 13.9% of the initial As concentration in contaminated soil after 63 days. Therefore, a synergistic degradation system is the most effective approach to degrade pyrene and remove arsenic in contaminated soil. These findings highlight the role of these strains in the bioremediation of environments contaminated with pyrene and arsenic.

Keywords pyrene arsenic bioremediation bacteria fungus

Corresponding Author(s): SUN Guoxin,Email:gxsun@rcees.ac.cn

Issue Date: 01 April 2013

Cite this article:

Shuang LIU,Guoxin SUN,Yanwei HOU. Synergistic degradation of pyrene and volatilization of arsenic by cocultures of bacteria and a fungus[J]. Front Envir Sci Eng, 2013, 7(2): 191-199.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-012-0470-3
https://academic.hep.com.cn/fese/EN/Y2013/V7/I2/191

Fig.1 Phylogenetic tree constructed from a comparative analysis of 16S rRNA gene sequences showing the relationships of of pyrene-degrading stains PY1 (a), PY2 (b), PY3 (c)

Tab.1 Profile of growth attained by individual bacterial and fungal culture in three different treatment regimes of pyrene and As 20 μM after 9 days of incubation

Fig.2 Removal of pyrene (100 mg·kg) and microbial growth by the individual bacteria and fungi: sp. PY1 (a), sp. PY2 (b), sp. PY3 (c) in the BSM. “b” represents sp. PY1, “s” represents sp. PY2, and “f” represents sp. PY3. Data shown are the mean values (±standard deviations) from four replications

Fig.3 Kinetic profiles for pyrene degradation by individual cultures ( sp. PY1, sp. PY2, sp. PY3), mixed bacterial cultures ( sp. PY1+ sp. PY2) and fungal-bacterial coculture ( sp. PY1+ sp. PY2+ sp. PY3) in the BSM containing pyrene (100 mg·kg). “b” represents sp. PY1, “s” represents sp. PY2, and “f” represents sp. PY3. Data shown are the mean values (±standard deviations) from four replications

Fig.4 Biodegradation of pyrene by microbial culture from contaminated soil: the pyrene concentration in the soil was 100 mg·kg. Microbial culture: individual cultures ( sp. PY1, sp. PY2, sp. PY3), mixed bacterial cultures ( sp. PY1+ sp. PY2) and fungal-bacterial coculture (sp. PY1+ . PY2+ sp. PY3) in the soil containing pyrene (100 mg·kg). “b” represents sp. PY1, “s” represents sp. PY2, and “f” represents sp. PY3. Data shown are the mean values (±standard deviations) from four replications

Fig.5 The sum of volatile As in the BSM by individual cultures ( sp. PY1, sp. PY2), mixed bacterial cultures ( sp PY1+ sp. PY2) and fungal-bacterial coculture ( sp. PY1+ sp. PY2+ sp. PY3) in the BSM containing pyrene (100 mg·kg) and As 20 μM. “b” represents sp. PY1, “s” represents sp. PY2, and “f” represents sp. PY3. Data shown are the mean values (±standard deviations) from four replications

Fig.6 The sum of volatile As in the soil by individual cultures ( sp. PY1, sp. PY2), mixed bacterial cultures ( sp. PY1+ sp. PY2) and fungal-bacterial coculture ( sp. PY1+ sp. PY2+ sp. PY3) in the soil containing pyrene (100 mg·kg) and arsenic (42 mg·kg). “b” represents sp. PY1, “s” represents sp. PY2, and “f” represents sp. PY3. Data shown are the mean values (±standard deviations) from four replications

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