In-situ</italic> mineralization of chitosan/calcium phosphate composite and the effect of solvent on the structure

doi:10.1007/s11706-011-0140-6

Front Mater Sci

2011, Vol. 5

Issue (3) : 282-292 https://doi.org/10.1007/s11706-011-0140-6

RESEARCH ARTICLE

In-situ mineralization of chitosan/calcium phosphate composite and the effect of solvent on the structure

Ling-Hao HE¹, Lu YAO¹, Rui XUE¹, Jing SUN¹, Rui SONG^1,2(

)

1. State Key Laboratory of Surface & Interface Science of Henan Provincial, Zhengzhou University of Light Industry, Zhengzhou 450002, China; 2. College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing 100049, China

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Abstract

Solvent played an important role in the formation of calcium phosphate phase of the chitosan/calcium phosphate composites. In this investigation, ethanol-acetic acid mixtures were employed as solvents, and various calcium phosphate phases, such as brushite, amorphous calcium phosphate, and hydroxyapatite, were introduced into the chitosan/calcium phosphate composites by using in-situ preparation process. The results showed that the structures of composite were influenced remarkably by the morphology and the distribution of calcium phosphate phase. In addition, the bioactivity of composites was governed mainly by the characters of calcium phosphate phases in composites, since calcium phosphate phases could induce the growth of hydroxyapatite coating on the surfaces of composites. On the surface of chitosan/brushite composite, the formed hydroxyapatite coating consisted of oriented plate crystallites, which self-assembled into spherical-like crystals. When other calcium phosphate phase was introduced into composites, the polymorphs of hydroxyapatite layer would change greatly. The oriented plate crystallites became bigger, and meanwhile, the self-assembled aggregates became less and smaller. In addition, with the shift of the prior nucleating point, the growth orientation of plate crystallites was transformed.

Keywords chitosan composite membrane hydroxyapatite ethanol

Corresponding Author(s): SONG Rui,Email:rsong@gucas.ac.cn

Issue Date: 05 September 2011

Cite this article:

Ling-Hao HE,Lu YAO,Rui XUE, et al. In-situ mineralization of chitosan/calcium phosphate composite and the effect of solvent on the structure[J]. Front Mater Sci, 2011, 5(3): 282-292.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-011-0140-6
https://academic.hep.com.cn/foms/EN/Y2011/V5/I3/282

Fig.1 Schematic illustration of the whole experimental procedure.

Tab.1 Synthetic conditions of the chitosan/calcium phosphate composites ( (I), mineralizing (M))

Fig.2 XRD patterns showing the presence of brushite in all composite membranes including samples 0-I, 30-I, and 50-I.

Fig.3 ATR-infrared spectra of composite membranes prepared in synthetic process by introducing ethanol into solutions with 0%, 30%, and 50% ratios of solvent, respectively.

Fig.4 Raman spectra of composite membranes prepared in synthetic process by introducing ethanol into solution with 0%, 30%, and 50% ratios of solvent, respectively.

Fig.5 SEM morphology (left panels) and optical microscope (right panels) of composite membranes prepared in preparing process by introducing ethanol into solution with sample 0-I, sample 30-I, and sample 50-I.

Tab.2 Relative viscosity of chitosan ethanol-acetic acid solution with different ethanol ratios

Fig.6 Schematic representation of the influence of ethanol concentration on the composite membrane structure (acidic chitosan solution served as body solution): In the absence of ethanol solvent, the formation of composite membrane structure yielded to acidic chitosan solution. In the presence of ethanol solvent with 30% content, the formed amorphous calcium phosphate influenced the aggregation growth of brushite. In the presence of ethanol solvent with 50% content, the phase separation of solution state was a key factor for the formation of composite membrane.

Fig.7 OM images of samples 0-I, 30-I, 50-I, and SEM images of samples 0-I, 30-I, and 50-I, showing morphologies of HAP coatings grown on the surface of composite membranes in SCS solution for 7 d.

Fig.8 ATR-infrared spectra of HAP coatings grown on the surface of composite membranes in SCS solution for 7 d.

Fig.9 XRD patterns of HAP coatings grown on the surface of composite membranes in the SCS solution for 7 d.

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