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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

Postal Subscription Code 80-974

2018 Impact Factor: 1.701

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Front. Mater. Sci.    2016, Vol. 10 Issue (3) : 270-280    https://doi.org/10.1007/s11706-016-0347-7
RESEARCH ARTICLE
Electrophoretic-deposited novel ternary silk fibroin/graphene oxide/hydroxyapatite nanocomposite coatings on titanium substrate for orthopedic applications
Ming LI1,3,Pan XIONG3,Maosong MO1,2,*(),Yan CHENG3,*(),Yufeng ZHENG3,4
1. Institute of Electrical Engineering , Chinese Academy of Sciences, Beijing 100190, China
2. School of Electronic, Electrical?and?Communication?Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
3. Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
4. Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China
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Abstract

The combination of graphene oxide (GO) with robust mechanical property, silk fibroin (SF) with fascinating biological effects and hydroxyapatite (HA) with superior osteogenic activity is a competitive approach to make novel coatings for orthopedic applications. Herein, the feasibility of depositing ternary SF/GO/HA nanocomposite coatings on Ti substrate was firstly verified by exploiting electrophoretic nanotechnology, with SF being used as both a charging additive and a dispersion agent. The surface morphology, microstructure and composition, in vitro hemocompatibility and in vitro cytocompatibility of the resulting coatings were investigated by SEM, Raman, FTIR spectra and biocompatibility tests. Results demonstrated that GO, HA and SF could be co-deposited with a uniform, smooth thin-film morphology. The hemolysis rate analysis and the platelet adhesion test indicated good blood compatibility of the coatings. The human osteosarcoma MG63 cells displayed well adhesion and proliferation behaviors on the prepared coatings, with enhanced ALP activities. The present study suggested that SF/GO/HA nanocomposite coatings could be a promising candidate for the surface functionalization of biomaterials, especially as orthopedic implant coating.
Keywords graphene oxide      silk fibroin      hydroxyapatite      nano-biocomposite coatings      electrodeposition     
Corresponding Author(s): Maosong MO,Yan CHENG   
Online First Date: 08 June 2016    Issue Date: 08 August 2016
 Cite this article:   
Ming LI,Pan XIONG,Maosong MO, et al. Electrophoretic-deposited novel ternary silk fibroin/graphene oxide/hydroxyapatite nanocomposite coatings on titanium substrate for orthopedic applications[J]. Front. Mater. Sci., 2016, 10(3): 270-280.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-016-0347-7
https://academic.hep.com.cn/foms/EN/Y2016/V10/I3/270
Coating V(silk)/mL a) V(GO)/mL b) m(HA)/mg V(DI)/mL V(ethanol)/mL
SF 15 0 0 25.5 5.5
SF/GO 15 1 0 24.5 5.5
SF/HA 15 0 24 25.5 5.5
SF/GO/HA 15 1 24 24.5 5.5
Tab.1  The composition of the suspensions for EPD
Fig.1  SEM images of pure (a)(e) SF, (b)(f) SF/GO, (c)(g) SF/HA and (d)(h) SF/GO/HA nanocomposite coatings on Ti substrate.
Fig.2  Raman spectra of electrophoretic deposited coatings on Ti substrate.
Fig.3  FTIR spectra of electrophoretic-deposited coatings on Ti substrate.
Coating E/GPa H/MPa E/H
SF 7.36±0.67 184.4±10.5 39.91
SF/GO 15.8±2.32 234.0±64.8 67.52
SF/HA 13.92±0.81 249.2±29.6 55.86
SF/GO/HA 21.28±0.29 293.1±26.4 72.60
Tab.2  The elastic modulus (E) , hardness (H) and the calculated E/H values of the coatings
Fig.4  SEM images of the adhered platelets on pristine (a) Ti, (b) SF, (c) SF/GO, (d) SF/HA and (e) SF/GO/HA coatings.
Fig.5  (a) SEM images of the adhered MG63 cells on pristine uncoated and coated Ti sheets after being cultured for 1, 4 and 7 d. (b) The cell viability and (c) normalized ALP activity of the MG63 cells after culturing of 7 d on the prepared samples.
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