Green synthesis of biocompatible gold nanoparticles using <italic>Fagopyrum esculentum</italic> leaf extract

doi:10.1007/s11706-011-0153-1

Front Mater Sci

2011, Vol. 5

Issue (4) : 379-387 https://doi.org/10.1007/s11706-011-0153-1

RESEARCH ARTICLE

Green synthesis of biocompatible gold nanoparticles using Fagopyrum esculentum leaf extract

Punuri Jayasekhar BABU^1,2, Pragya SHARMA³, Mohan Chandra KALITA³, Utpal BORA^1,2(

)

1. Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam-781039, India; 2. Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam-781039, India; 3. Department of Biotechnology, Gauhati University, Guwahati, Assam-781039, India

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Abstract

This report describes the use of ethnolic extract of Fagopyrum esculentum leaves for the synthesis of gold nanoparticles. UV-visible spectroscopy analysis indicated the successful formation of gold nanoparticles. The synthesized nanoparticles were characterized by transmission electron microscopy (TEM), high resolution TEM (HRTEM) and were found to be spherical, hexagonal and triangular in shape with an average size of 8.3 nm. The crystalline nature of the gold nanoparticles was confirmed from X-ray diffraction (XRD) and selected-area electron diffraction (SAED) patterns. Fourier transform infrared (FT-IR) and energy-dispersive X-ray analysis (EDX) suggested the presence of organic biomolecules on the surface of the gold nanoparticles. Cytotoxicity tests against human HeLa, MCF-7 and IMR-32 cancer cell lines revealed that the gold nanoparticles were non-toxic and thus have potential for use in various biomedical applications.

Keywords biomaterials crystal growth electron microscopy Fourier transform infrared spectroscopy adsorption

Corresponding Author(s): BORA Utpal,Email:ubora@iitg.ernet.in, ubora@rediffmail.com

Issue Date: 05 December 2011

Cite this article:

Punuri Jayasekhar BABU,Pragya SHARMA,Mohan Chandra KALITA, et al. Green synthesis of biocompatible gold nanoparticles using Fagopyrum esculentum leaf extract[J]. Front Mater Sci, 2011, 5(4): 379-387.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-011-0153-1
https://academic.hep.com.cn/foms/EN/Y2011/V5/I4/379

Fig.1 UV-visible absorption spectra of GNPs synthesized by different concentrations of FLE (a —0.1%, b — 0.2%, c —0.3%, d —0.4%, e —0.5%) for 15 s against 1 mmol/L HAuCl and at varying MW irradiation time (a—12 s, b —14 s, c — 16 s, d —18 s, e— 20 s) with 0.4% FLE and 1 mmol/L HAuCl.

Fig.2 Tentative mechanisms of flavonoids and phenolic compounds.

Fig.3 TEM image of GNPs synthesized with 0.4% and 1 mmol/L HAuCl for 16 s; SAED pattern; HRTEM and size distribution histograms.

Fig.4 XRD pattern of GNPs synthesized with 0.4% FLE, 1 mmol/L HAuCl and 16 s as MW irradiation time.

Fig.5 FT-IR spectra of lyophilized FLE (a) and GNPs (b).

Fig.6 H NMR spectra of GNPs.

Fig.7 EDX pattern of GNPs synthesized with 0.4% FLE, 1 mmol/L HAuCl and 16 s MW irradiation time.

Fig.8 Cytotoxicity assay: cell viability of HeLa, MCF-7 and IMR-32 cells exposed to different concentrations of GNPs (10-100 μmol/L) over a period of 24 h treatment.

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