Surface modification of apatite--wollastonite glass ceramic by synthetic coupling agent

doi:10.1007/s11706-014-0244-x

Front. Mater. Sci.

2014, Vol. 8

Issue (2) : 157-164 https://doi.org/10.1007/s11706-014-0244-x

RESEARCH ARTICLE

Surface modification of apatite--wollastonite glass ceramic by synthetic coupling agent

Qin LONG¹,Da-Li ZHOU^2,^*(

),Xiang ZHANG^2,³,Jia-Bei ZHOU¹

1. College of Chemical Engineering, Sichuan University, Chengdu 610065, China
2. College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China
3. Gannan Medical University, Ganzhou 341000, China

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Abstract

In this study, lysine was introduced into the surface of apatite--wollastonite glass ceramic (AW-GC) to improve its cytocompatibility by two steps reaction procedure. Firstly, lysine connected to N-β-(aminoethyl)-γ-aminopropyl trimethoxy silane (A-1120) by covalent binding of amide group. Secondly, the lysine-functionalized A-1120 was deposited on the surface of AW-GC through a silanization reaction involving a covalent attachment. FTIR spectra indicated that lysine had been immobilized onto the surface of AW-GC successfully. Bioactivity of the surface modified AW-GC was investigated by simulated body fluid (SBF), and the in vitro cytocompatibility was evaluated by co-culturing with human osteosarcoma cell MG63. The results showed that the process of hydroxyapatite layer formed on the modified material was similar to AW-GC while the mode of hydroxyapatite deposition was changed. The growth of MG63 cells showed that modifying the AW-GC surface with lysine enhances the cell adhesion and proliferation.

Keywords surface modification apatite--wollastonite glass ceramic (AW-GC) lysine silane coupling agent cytocompatibility

Corresponding Author(s): Da-Li ZHOU

Issue Date: 24 June 2014

Cite this article:

Qin LONG,Da-Li ZHOU,Xiang ZHANG, et al. Surface modification of apatite--wollastonite glass ceramic by synthetic coupling agent[J]. Front. Mater. Sci., 2014, 8(2): 157-164.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-014-0244-x
https://academic.hep.com.cn/foms/EN/Y2014/V8/I2/157

Fig.1 Scheme 1 Preparation of silane coupling agent and surface modification of AW-GC.

Fig.2 FTIR spectra of (a) AW-GC and (b) S-AW.

Fig.3 SEM images of (a) AW-GC and (b) S-AW.

Fig.4 SEM images of (a) S-AW soaked in SBF for 4 d, (b) S-AW soaked in SBF for 7 d, (c) S-AW soaked in SBF for 14 d, and (d) AW-GC soaked in SBF for 4 d.

Fig.5 MTT assay of materials after different times of cell culture (* p<0.05 Differences between test groups were considered statistically significant).

Fig.6 SEM images of (a) pure AW-GC/cell co-cultured for 3 d, (b) AW-GC surface modified by A-1120/cell co-cultured for 3 d, and (c) S-AW/cell co-cultured for 3 d.

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