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Effect of Mg 2+ concentration
on biocompatibility of pure magnesium |
| Jia-Cheng GAO1,Li-Ying QIAO1,Ren-Long XIN2, |
| 1.National Engineering
Center for Magnesium Alloy, Chongqing University, Chongqing 400044,
China；College of Material
Science and Engineering, Chongqing University, Chongqing 400045, China; 2.College of Material
Science and Engineering, Chongqing University, Chongqing 400045, China; |
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Abstract The aim of the present study is to find the correlation between the Mg2+ concentration degraded from pure magnesium material and the biocompatibility of the material. Hemolysis ratio (HR) of the extracts of pure magnesium with different Mg2+ concentration were measured according to ISO 10993.4 standard. The cytotoxicity tests were carried out by both indirect contact with fibroblast L929 and preosteoblasts MC3T3-E1, and MTT tests were used. Cytotoxicity of the pure magnesium with and without surface modification was further evaluated by direct contact method. Samples were cultured with Osteoblast MC3T3-E1 and the effects of the material on viability and activity of cells were discussed. The results showed that the hemolysis rate and cytotoxicity of the modified Mg could meet the requirement for biomaterials. In our test, the hemolysis rate of the extracts was qualified when the concentration of Mg2+£ 42 mg/L; the extracts with 202 mg/L Mg2+ met the cytotoxicity requirement, and the extracts with 156 mg/L Mg2+ promoted cell proliferation.€Therefore, the€biocompatibili-ty of magnesium-based materials can be improved by suitable surface modification.
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| Keywords
magnesium
biomaterials
surface modification
biocompatibility
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Issue Date: 05 June 2010
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McBride E D. Absorbable metal in bone surgery. Journal of the American Medical Association, 1938, 111(27): 2464–2467
|
|
McCord C P. Chemical gas gangrene from metallic magnesium. Industrial Medicine, 1942, 1(2): 71–79
|
|
Kuwahara H, Al-Abdullat Y, Ohta M, et al. Surface reactionof magnesium in Hank’s solutions. Materials Science Forum, 2000, 350―351: 349–358
doi: 10.4028/www.scientific.net/MSF.350-351.349
|
|
Gao J C, Wu S, Qiao L Y, et al. Corrosion behavior of magnesiumand its alloy in simulated body fluid. Journal of Clinical Rehabilitative Tissue Engineering Research, 2007, 11(18): 3584–3586 (in Chinese)
|
|
Gao J-C, Wu S, Qiao L-Y, et al. Corrosion behavior of Mg andMg-Zn alloys in simulated body fluid. Transactions of Nonferrous Metals Society of China, 2008, 18(3): 588–592
doi: 10.1016/S1003-6326(08)60102-8
|
|
Muller W D, Nascimento M L, Zeddies M, et al. Magnesium and itsalloys as degradable biomaterials. Corrosion studies using potentiodynamicand EIS electrochemical techniques. Materials Research, 2007, 10(1): 5–10
doi: 10.1590/S1516-14392007000100003
|
|
Kuwahara H, Al-Abdullat Y, Mazaki N, et al. Precipitation ofmagnesium apatite on pure magnesium surface during immersing in Hank’ssolution. Materials Transactions, 2001, 42(7): 1317–1321
doi: 10.2320/matertrans.42.1317
|
|
Li L, Gao J, Wang Y. Evaluation of cyto-toxicity and corrosionbehavior of alkali-treated magnesium in simulated body fluid. Surface and Coatings Technology, 2004, 185(1): 92–98
doi: 10.1016/j.surfcoat.2004.01.004
|
|
Gao J, Xue Y, Qiao L, et al. Surface modification of magnesiumwith rare earth conversion films for biomedical protection. Materials Science Forum, 2007, 546―549: 601–604
|
|
Zhang E, Xu L, Yang K. Formation by ion plating of Ti-coatingon pure Mg for biomedical applications. Scripta Materialia, 2005, 53(5): 523–527
doi: 10.1016/j.scriptamat.2005.05.009
|
|
Liu C, Xin Y, Tian X, et al. Corrosion resistance of titaniumion implanted AZ91 magnesium alloy. Journal of Vacuum Science and Technology A, 2007, 25(2): 334–339
doi: 10.1116/1.2699371
|
|
Liu C, Xin Y, Tian X, et al. Corrosion behavior of AZ91 magnesiumalloy treated by plasma immersion ion implantation and depositionin artificial physiological fluids. Thin Solid Films, 2007, 516(2―4): 422–427
doi: 10.1016/j.tsf.2007.05.048
|
|
Li Z, Gu X, Lou S, et al. The development of binary Mg-Caalloys for use as biodegradable materials within bone. Biomaterials, 2008, 29(10): 1329–1344
doi: 10.1016/j.biomaterials.2007.12.021
|
|
Wu J. Biocompatibility and Micro-arc-oxidation Surface Treatmentof AZ91D Magnesium Alloy. Nanjing: Stomatological Hospital of Nanjing Medical University, 2008 (in Chinese)
|
|
ISO 10993-12. Biological Evaluation of Medical Devices-Part 12: SamplePreparation and Reference Materials, 1996
|
|
ISO 10993-4. Biological Evaluation of Medical Devices-Part 4: Selectionof Tests for Interactions with Blood, Annex D, 1993
|
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