Hydrothermal growth of hydroxyapatite and ZnO bilayered nanoarrays on magnesium alloy surface with antibacterial activities

doi:10.1007/s11706-020-0489-5

Front. Mater. Sci.

2020, Vol. 14

Issue (1) : 14-23 https://doi.org/10.1007/s11706-020-0489-5

RESEARCH ARTICLE

Hydrothermal growth of hydroxyapatite and ZnO bilayered nanoarrays on magnesium alloy surface with antibacterial activities

Mengke PENG¹, Fenyan HU¹, Minting DU¹, Bingjie MAI², Shurong ZHENG¹, Peng LIU³, Changhao WANG¹(

), Yashao CHEN¹(

)

¹. Key Laboratory of Applied Surface and Colloid Chemistry (Ministry of Education), School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710119, China
². Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Ministry of Education), National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China
³. Key Laboratory of Biorheological Science and Technology of Ministry of Education, Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, College of Bioengineering, Chongqing University, Chongqing 400044, China

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Abstract

Magnesium alloy (MgA) has been extensively used as orthopedic and cardiovascular scaffolds in virtue of its good biocompatibility, unique biodegradability and excellent mechanical properties. However, poor corrosion resistance and easy infection after implantation seriously limit the potential applications of MgA in the biomedical field. Herein, we fabricated bilayered nanoarrays of hydroxyapatite nanorods (HANRs) and ZnO nanorods (ZnONRs) onto the surface of MgA (MgA–MgO–HANRs– ZnONRs) via micro-arc oxidation (MAO) treatment, microwave-assisted hydrothermal and hydrothermal methods. The morphology and chemical composition of MgA–MgO– HANRs–ZnONRs was characterized by FE-SEM, XRD and EDS, indicating that HANRs–ZnONRs bilayered nanoarrays were fabricated on the surface of MgA–MgO. The surface of MgA–MgO–HANRs–ZnONRs exhibited excellent hydrophilicity as evidenced by the low water contact angle of 3°. Compared with the original MgA, the corrosion resistance of MgA–MgO–HANRs–ZnONRs was obviously improved with decreasing the corrosive current density (i_corr) of 2 orders of magnitude. The MgA–MgO– HANRs–ZnONRs performed excellent antibacterial properties with the bactericidal rate of 96.5% against S. aureus and 94.3% against E. coli.

Keywords magnesium alloy nanoarray hydrothermal synthesis antibacterial activity corrosion resistance

Corresponding Author(s): Changhao WANG,Yashao CHEN

Online First Date: 27 December 2019 Issue Date: 05 March 2020

Cite this article:

Mengke PENG,Fenyan HU,Minting DU, et al. Hydrothermal growth of hydroxyapatite and ZnO bilayered nanoarrays on magnesium alloy surface with antibacterial activities[J]. Front. Mater. Sci., 2020, 14(1): 14-23.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-020-0489-5
https://academic.hep.com.cn/foms/EN/Y2020/V14/I1/14

Fig.1 Schematic representation of the working flow for preparing MgA?MgO?HANRs?ZnONRs.

Fig.2 FE-SEM images of (a) MgA, (b) MgA?MgO, (c) MgA?MgO?HANRs and (d) MgA?MgO?HANRs?ZnONRs. (e) FE-SEM image and (f) EDS elemental mappings for cross-section of MgA?MgO?HANRs?ZnONRs.

Fig.3 FTIR spectra of MgA (a), MgA?MgO (b), MgA?MgO?HANRs (c) and MgA?MgO?HANRs?ZnONRs (d).

Fig.4 XRD patterns of MgA (a), MgA?MgO (b), MgA?MgO?HANRs (c) and MgA?MgO?HANRs?ZnONRs (d).

Fig.5 Water contact angles of (a) MgA, (b) MgA?MgO, (c) MgA?MgO?HANRs and (d) MgA?MgO?HANRs?ZnONRs.

Fig.6 Potentiodynamic polarization curves of MgA (a), MgA?MgO (b), MgA?MgO?HANRs (c) and MgA?MgO?HANRs?ZnONRs (d).

Tab.1 The corrosion potential (E_corr) and corrosion current (i_corr) of different MgA samples

Fig.7 E.coli and S. aureus formed on LB agar plates and antibacterial rate with different MgA samples: (a) MgA; (b) MgA?MgO?HANRs; (c) MgA?MgO?HANRs?ZnONRs.

Fig. S1 EDS spectra of (a) MgA, (b) MgA?MgO, (c) MgA?MgO?HANRs and (d) MgA?MgO?HANRs?ZnONRs.

Table S1 The chemical composition of different MgA samples

Fig. S2 FE-SEM images of E. coli and S. aureus (a) before and (b) after treatment with MgA?MgO?HANRs?ZnONRs.

Fig. S3(a) Zn²⁺ release profile of MgA?MgO?HANRs?ZnONRs in PBS buffer. (b)(c)(d) FE-SEM images of MgA?MgO?HANRs?ZnONRs in PBS buffer at different time. (e) EDS result.

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