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Protein & Cell

ISSN 1674-800X

ISSN 1674-8018(Online)

CN 11-5886/Q

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Prot Cell    2012, Vol. 3 Issue (10) : 790-801    https://doi.org/10.1007/s13238-012-2069-7      PMID: 23055042
RESEARCH ARTICLE
Human Bop is a novel BH3-only member of the Bcl-2 protein family
Xiaoping Zhang1,3, Changjiang Weng1, Yuan Li1,3, Xiaoyan Wang1,4, Chunsun Jiang2, Xuemei Li1, Youli Xu1, Quan Chen2, Lei Pan1(), Hong Tang1()
1. The Key Laboratory of Infection and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2. National Key Laboratory of Bio-membrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 3. The Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 4. Taizhou Institute of Protein Engineering, Taizhou National Biomedicine Hightech Park, Jiangsu 225300, China
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Abstract

One group of Bcl-2 protein family, which shares only the BH3 domain (BH3-only), is critically involved in the regulation of programmed cell death. Herein we demonstrated a novel human BH3-only protein (designated as Bop) which could induce apoptosis in a BH3 domain-dependent manner. Further analysis indicated that Bop mainly localized to mitochondria and used its BH3 domain to contact the loop regions of voltage dependent anion channel 1 (VDAC1) in the outer mitochondrial membrane. In addition, purified Bop protein induced the loss of mitochondrial transmembrane potential (ΔΨm) and the release of cytochrome c. Furthermore, Bop used its BH3 domain to contact pro-survival Bcl-2 family members (Bcl-2, Bcl-XL, Mcl-1, A1 and Bcl-w), which could inhibit Bop-induced apoptosis. Bop would be constrained by pro-survival Bcl-2 proteins in resting cells, because Bop became released from phosphorylated Bcl-2 induced by microtubule-interfering agent like vincristine (VCR). Indeed, knockdown experiments indicated that Bop was partially required for VCR induced cell death. Finally, Bop might need to function through Bak and Bax, likely by releasing Bak from Bcl-XL sequestration. In conclusion, Bop may be a novel BH3-only factor that can engage with the regulatory network of Bcl-2 family members to process intrinsic apoptotic signaling.
Keywords apoptosis      BH3 domain      Bop     
Corresponding Author(s): Pan Lei,Email:panlei@moon.ibp.ac.cn; Tang Hong,Email:tanghong@moon.ibp.ac.cn   
Issue Date: 01 October 2012
 Cite this article:   
Xuemei Li,Youli Xu,Quan Chen, et al. Human Bop is a novel BH3-only member of the Bcl-2 protein family[J]. Prot Cell, 2012, 3(10): 790-801.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1007/s13238-012-2069-7
https://academic.hep.com.cn/pac/EN/Y2012/V3/I10/790
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