Acid Orange 7 degradation using methane as the sole carbon source and electron donor

doi:10.1007/s11783-021-1468-5

Front. Environ. Sci. Eng.

2022, Vol. 16

Issue (3) : 34 https://doi.org/10.1007/s11783-021-1468-5

RESEARCH ARTICLE

Acid Orange 7 degradation using methane as the sole carbon source and electron donor

Yanan Bai^1,^2,³, Xiuning Wang⁴, Fang Zhang², Raymond Jianxiong Zeng^2,^3,⁴(

)

¹. School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China
². Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
³. Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou 215123, China
⁴. CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, China

Download: PDF(982 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract

• AO7 degradation was coupled with anaerobic methane oxidation.

• Higher concentration of AO7 inhibited the degradation.

• The maximum removal rate of AO7 reached 280 mg/(L·d) in HfMBR.

• ANME-2d dominated the microbial community in both batch reactor and HfMBR.

• ANME-2d alone or synergistic with the partner bacteria played a significant role.

Azo dyes are widely applied in the textile industry but are not entirely consumed during the dyeing process and can thus be discharged to the environment in wastewater. However, azo dyes can be degraded using various electron donors, and in this paper, Acid Orange 7 (AO7) degradation performance is investigated using methane (CH₄) as the sole electron donor. Methane has multiple sources and is readily available and inexpensive. Experiments using ¹³C-labeled isotopes showed that AO7 degradation was coupled with anaerobic oxidation of methane (AOM) and, subsequently, affected by the initial concentrations of AO7. Higher concentrations of AO7 could inhibit the activity of microorganisms, which was confirmed by the long-term performance of AO7 degradation, with maximum removal rates of 8.94 mg/(L·d) in a batch reactor and 280 mg/(L·d) in a hollow fiber membrane bioreactor (HfMBR). High-throughput sequencing using 16S rRNA genes showed that Candidatus Methanoperedens, affiliated to ANME-2d, dominated the microbial community in the batch reactor and HfMBR. Additionally, the relative abundance of Proteobacteria bacteria (Phenylobacterium, Pseudomonas, and Geothermobacter) improved after AO7 degradation. This outcome suggested that ANME-2d alone, or acting synergistically with partner bacteria, played a key role in the process of AO7 degradation coupled with AOM.

Keywords Azo dyes AO7 degradation Anaerobic methane oxidation Microbial community ANME-2d

Corresponding Author(s): Raymond Jianxiong Zeng

Issue Date: 13 July 2021

Cite this article:

Yanan Bai,Xiuning Wang,Fang Zhang, et al. Acid Orange 7 degradation using methane as the sole carbon source and electron donor[J]. Front. Environ. Sci. Eng., 2022, 16(3): 34.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-021-1468-5
https://academic.hep.com.cn/fese/EN/Y2022/V16/I3/34

Fig.1 AO7 degradation coupled with methane oxidation in the methane group (AO7-¹³CH₄) and nitrogen group (AO7-N₂), and the ratio of ¹³CO₂/(¹²CO₂+¹³CO₂) in the isotope tracer experiment.

Fig.2 AO7 degradation over six days with different initial AO7 concentrations (4, 8, 16, 25, 50, 100, and 160 mg/L).

Fig.3 (a) UV–Vis absorption spectra before and after AO7 degradation; (b) stoichiometric relationship between AO7 and ABA on day 0 and day 10.

Fig.4 AO7 degradation performance in batch experiments, expressed as AO7 concentration and AO7 removal rate.

Fig.5 (a) AO7 degradation performance of the HfMBR in the start-up stage; (b) The influent and effluent AO7 concentration in the continuous-flow stage; (c) AO7 removal rate, efficiency and loading rate in the continuous-flow stage.

Tab.1 Microbial community richness and Alpha diversity of samples in the inoculum, batch reactor, and HfMBR

Fig.6 Relative abundances of microorganisms in the inoculum, batch reactor, and HfMBR. (a) Phylum; (b) Genus.

1	Y N Bai, X N Wang, J Wu, Y Z Lu, L Fu, F Zhang, T C Lau, R J Zeng (2019). Humic substances as electron acceptors for anaerobic oxidation of methane driven by ANME-2d. Water Research, 164: 114935 https://doi.org/10.1016/j.watres.2019.114935
2	Y N Bai, X N Wang, F Zhang, J Wu, W Zhang, Y Z Lu, L Fu, T C Lau, R J Zeng (2020). High-rate anaerobic decolorization of methyl orange from synthetic azo dye wastewater in a methane-based hollow fiber membrane bioreactor. Journal of Hazardous Materials, 388: 121753 https://doi.org/10.1016/j.jhazmat.2019.121753
3	R Brás, A Gomes, M I Ferra, H M Pinheiro, I C Goncalves (2005). Monoazo and diazo dye decolourisation studies in a methanogenic UASB reactor. Journal of Biotechnology, 115(1): 57–66 https://doi.org/10.1016/j.jbiotec.2004.08.001
4	C Cai, Y Shi, J Guo, G W Tyson, S Hu, Z Yuan (2019). Acetate production from anaerobic oxidation of methane via intracellular storage compounds. Environmental Science & Technology, 53(13): 7371–7379 https://doi.org/10.1021/acs.est.9b00077
5	H Chen, L Zhao, S H Hu, Z G Yuan, J H Guo (2018). High-Rate production of short-chain fatty acids from methane in a mixed-culture membrane biofilm reactor. Environmental Science & Technology Letters, 5(11): 662–667 https://doi.org/10.1021/acs.estlett.8b00460
6	N Dafale, L Agrawal, A Kapley, S Meshram, H Purohit, S Wate (2010). Selection of indicator bacteria based on screening of 16S rDNA metagenomic library from a two-stage anoxic-oxic bioreactor system degrading azo dyes. Bioresource Technology, 101(2): 476–484 https://doi.org/10.1016/j.biortech.2009.08.006
7	R Dai, X Chen, Y Luo, P Ma, S Ni, X Xiang, G Li (2016). Inhibitory effect and mechanism of azo dyes on anaerobic methanogenic wastewater treatment: Can redox mediator remediate the inhibition? Water Research, 104: 408–417 https://doi.org/10.1016/j.watres.2016.08.046
8	J Ding, Y Z Lu, L Fu, Z W Ding, Y Mu, S H Cheng, R J Zeng (2017). Decoupling of DAMO archaea from DAMO bacteria in a methane-driven microbial fuel cell. Water Research, 110: 112–119 https://doi.org/10.1016/j.watres.2016.12.006
9	K F Ettwig, B Zhu, D Speth, J T Keltjens, M S M Jetten, B Kartal (2016). Archaea catalyze iron-dependent anaerobic oxidation of methane. Proceedings of the National Academy of Sciences of the United States of America, 113(45): 12792–12796 https://doi.org/10.1073/pnas.1609534113
10	E Fernando, T Keshavarz, G Kyazze (2014). Complete degradation of the azo dye Acid Orange-7 and bioelectricity generation in an integrated microbial fuel cell, aerobic two-stage bioreactor system in continuous flow mode at ambient temperature. Bioresource Technology, 156: 155–162 https://doi.org/10.1016/j.biortech.2014.01.036
11	L Fu, Y N Bai, Y Z Lu, J Ding, D Zhou, R J Zeng (2019). Degradation of organic pollutants by anaerobic methane-oxidizing microorganisms using methyl orange as example. Journal of Hazardous Materials, 364: 264–271 https://doi.org/10.1016/j.jhazmat.2018.10.036
12	L Fu, S W Li, Z W Ding, J Ding, Y Z Lu, R J Zeng (2016). Iron reduction in the DAMO/Shewanella oneidensis MR-1 coculture system and the fate of Fe(II). Water Research, 88: 808–815 https://doi.org/10.1016/j.watres.2015.11.011
13	D Georgiou, A Aivasidis (2006). Decoloration of textile wastewater by means of a fluidized-bed loop reactor and immobilized anaerobic bacteria. Journal of Hazardous Materials, 135(1–3): 372–377 https://doi.org/10.1016/j.jhazmat.2005.11.081
14	I C Gonçalves, L Lopes, H M Pinheiro, M I Ferra (2009). Behaviour of different anaerobic populations on the biodegradation of textile chemicals. Journal of Hazardous Materials, 172(2–3): 1236–1243 https://doi.org/10.1016/j.jhazmat.2009.07.141
15	M F Haroon, S Hu, Y Shi, M Imelfort, J Keller, P Hugenholtz, Z Yuan, G W Tyson (2013). Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage. Nature, 500(7464): 567–570 https://doi.org/10.1038/nature12375
16	K Ilić Đurđić, R Ostafe, O Prodanović, A Đurđević Đelmaš, N Popović, R Fischer, S Schillberg, R Prodanović (2021). Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls. Frontiers of Environmental Science & Engineering, 15(2): 19 https://doi.org/10.1007/s11783-020-1311-4
17	M Işık, D T Sponza (2004). Decolorization of azo dyes under batch anaerobic and sequential anaerobic/aerobic conditions. Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances & Environmental Engineering, 39(4): 1107–1127 https://doi.org/10.1081/ESE-120028417
18	D C Kalyani, P S Patil, J P Jadhav, S P Govindwar (2008). Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresource Technology, 99(11): 4635–4641 https://doi.org/10.1016/j.biortech.2007.06.058
19	I K Kapdan, M Tekol, F Sengul (2003). Decolorization of simulated textile wastewater in an anaerobic–aerobic sequential treatment system. Process Biochemistry, 38(7): 1031–1037 https://doi.org/10.1016/S0032-9592(02)00238-8
20	K Knittel, A Boetius (2009). Anaerobic oxidation of methane: progress with an unknown process. Annual Review of Microbiology, 63(1): 311–334 https://doi.org/10.1146/annurev.micro.61.080706.093130
21	Y Liu, Y Zhang, X Quan, J Zhang, H Zhao, S Chen (2011). Effects of an electric field and zero valent iron on anaerobic treatment of azo dye wastewater and microbial community structures. Bioresource Technology, 102(3): 2578–2584 https://doi.org/10.1016/j.biortech.2010.11.109
22	Y N Liu, F Zhang, J Li, D B Li, D F Liu, W W Li, H Q Yu (2017). Exclusive extracellular bioreduction of methyl orange by azo reductase-free Geobacter sulfurreducens. Environmental Science & Technology, 51(15): 8616–8623 https://doi.org/10.1021/acs.est.7b02122
23	Y Z Lu, Z W Ding, J Ding, L Fu, R J Zeng (2015). Design and evaluation of universal 16S rRNA gene primers for high-throughput sequencing to simultaneously detect DAMO microbes and anammox bacteria. Water Research, 87: 385–394 https://doi.org/10.1016/j.watres.2015.09.042
24	Y Z Lu, L Fu, J Ding, Z W Ding, N Li, R J Zeng (2016). Cr(VI) reduction coupled with anaerobic oxidation of methane in a laboratory reactor. Water Research, 102: 445–452 https://doi.org/10.1016/j.watres.2016.06.065
25	J H Luo, H Chen, S Hu, C Cai, Z Yuan, J Guo (2018). Microbial selenate reduction driven by a denitrifying anaerobic methane oxidation biofilm. Environmental Science & Technology, 52(7): 4006–4012 https://doi.org/10.1021/acs.est.7b05046
26	J H Luo, M Wu, J Liu, G Qian, Z Yuan, J Guo (2019). Microbial chromate reduction coupled with anaerobic oxidation of methane in a membrane biofilm reactor. Environment International, 130: 104926 https://doi.org/10.1016/j.envint.2019.104926
27	Y H Luo, R Chen, L L Wen, F Meng, Y Zhang, C Y Lai, B E Rittmann, H P Zhao, P Zheng (2015). Complete perchlorate reduction using methane as the sole electron donor and carbon source. Environmental Science & Technology, 49(4): 2341–2349 https://doi.org/10.1021/es504990m
28	S Mahmood, A Khalid, M Arshad, T Mahmood, D E Crowley (2016). Detoxification of azo dyes by bacterial oxidoreductase enzymes. Critical Reviews in Biotechnology, 36(4): 639–651 https://doi.org/10.3109/07388551.2015.1004518
29	B Manu, S Chaudhari (2002). Anaerobic decolorisation of simulated textile wastewater containing azo dyes. Bioresource Technology, 82(3): 225–231 https://doi.org/10.1016/S0960-8524(01)00190-0
30	D Méndez-Paz, F Omil, J M Lema (2005a). Anaerobic treatment of azo dye Acid Orange 7 under fed-batch and continuous conditions. Water Research, 39(5): 771–778 https://doi.org/10.1016/j.watres.2004.11.022
31	D Méndez-Paz, F Omil, J M Lema (2005b). Anaerobic treatment of azo dye Acid Orange 7 under batch conditions. Water Research, 36(2): 264–272
32	W B Nie, J Ding, G J Xie, X Tan, Y Lu, L Peng, B F Liu, D F Xing, Z Yuan, N Ren (2021). Simultaneous nitrate and sulfate dependent anaerobic oxidation of methane linking carbon, nitrogen and sulfur cycles. Water Research, 194: 116928 https://doi.org/10.1016/j.watres.2021.116928
33	Y S Oon, S A Ong, L N Ho, Y S Wong, Y L Oon, H K Lehl, W E Thung, N Nordin (2018). Disclosing the synergistic mechanisms of azo dye degradation and bioelectricity generation in a microbial fuel cell. Chemical Engineering Journal, 344: 236–245 https://doi.org/10.1016/j.cej.2018.03.060
34	A A Raghoebarsing, A Pol, K T Van De Pas-Schoonen, A J Smolders, K F Ettwig, W I Rijpstra, S Schouten, J S Damste, H J Op Den Camp, M S Jetten, M Strous (2006). A microbial consortium couples anaerobic methane oxidation to denitrification. Nature, 440(7086): 918–921 https://doi.org/10.1038/nature04617
35	Z J Ren (2017). Microbial fuel cells: Running on gas. Nature Energy, 2(6): 17093 https://doi.org/10.1038/nenergy.2017.93
36	R G Saratale, G D Saratale, J S Chang, S P Govindwar (2011). Bacterial decolorization and degradation of azo dyes: A review. Journal of the Taiwan Institute of Chemical Engineers, 42(1): 138–157 https://doi.org/10.1016/j.jtice.2010.06.006
37	P H Timmers, C U Welte, J J Koehorst, C M Plugge, M S Jetten, A J Stams (2017). Reverse Methanogenesis and Respiration in Methanotrophic Archaea. Archaea (Vancouver, B.C.), 2017: 1654237 https://doi.org/10.1155/2017/1654237
38	F P van Der Zee, S Villaverde (2005). Combined anaerobic-aerobic treatment of azo dyes: A short review of bioreactor studies. Water Research, 39(8): 1425–1440 https://doi.org/10.1016/j.watres.2005.03.007
39	A J Wallenius, P Dalcin Martins, C P Slomp, M S M Jetten (2021). Anthropogenic and environmental constraints on the microbial methane cycle in coastal sediments. Frontiers in Microbiology, 12: 631621
40	Z Wang, Q Yin, M Gu, K He, G Wu (2018). Enhanced azo dye Reactive Red 2 degradation in anaerobic reactors by dosing conductive material of ferroferric oxide. Journal of Hazardous Materials, 357: 226–234 https://doi.org/10.1016/j.jhazmat.2018.06.005
41	J Willetts, N J Ashbolt (2000). Understanding anaerobic decolourisation of textile dye wastewater: Mechanism and kinetics. Water Science and Technology, 42(1–2): 409–415 https://doi.org/10.2166/wst.2000.0347
42	S Xu, X Wu, H Lu (2021). Overlooked nitrogen-cycling microorganisms in biological wastewater treatment. Frontiers of Environmental Science & Engineering, 15(6): 133 https://doi.org/10.1007/s11783-021-1426-2
43	L Yu, X Y Zhang, S Wang, Q W Tang, T Xie, N Y Lei, Y L Chen, W C Qiao, W W Li, M H W Lam (2015). Microbial community structure associated with treatment of azo dye in a start-up anaerobic sequenced batch reactor. Journal of the Taiwan Institute of Chemical Engineers, 54: 118–124 https://doi.org/10.1016/j.jtice.2015.03.012

[1]

FSE-21053-OF-BYN_suppl_1

Download

[1]	Cheng Hou, Xinbai Jiang, Na Li, Zhenhua Zhang, Qian Zhang, Jinyou Shen, Xiaodong Liu. Enhanced 4-chlorophenol biodegradation by integrating Fe₂O₃ nanoparticles into an anaerobic reactor: Long-term performance and underlying mechanism[J]. Front. Environ. Sci. Eng., 2022, 16(8): 98-.
[2]	Yan Guo, Zibin Luo, Junhao Shen, Yu-You Li. The main anammox-based processes, the involved microbes and the novel process concept from the application perspective[J]. Front. Environ. Sci. Eng., 2022, 16(7): 84-.
[3]	Zecong Yu, Keke Xiao, Yuwei Zhu, Mei Sun, Sha Liang, Jingping Hu, Huijie Hou, Bingchuan Liu, Jiakuan Yang. Comparison of different valent iron on anaerobic sludge digestion: Focusing on oxidation reduction potential, dissolved organic nitrogen and microbial community[J]. Front. Environ. Sci. Eng., 2022, 16(6): 80-.
[4]	Qiong Guo, Zhichao Yang, Bingliang Zhang, Ming Hua, Changhong Liu, Bingcai Pan. Enhanced methane production during long-term UASB operation at high organic loads as enabled by the immobilized Fungi[J]. Front. Environ. Sci. Eng., 2022, 16(6): 71-.
[5]	Zaishan Wei, Meiru Tang, Zhenshan Huang, Huaiyong Jiao. Mercury removal from flue gas using nitrate as an electron acceptor in a membrane biofilm reactor[J]. Front. Environ. Sci. Eng., 2022, 16(2): 20-.
[6]	Shuhan Li, Xin Zhou, Xiwei Cao, Jiabo Chen. Insights into simultaneous anammox and denitrification system with short-term pyridine exposure: Process capability, inhibition kinetics and metabolic pathways[J]. Front. Environ. Sci. Eng., 2021, 15(6): 139-.
[7]	Qinxue Wen, Shuo Yang, Zhiqiang Chen. Mesophilic and thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbial communities and evolution of resistance genes[J]. Front. Environ. Sci. Eng., 2021, 15(5): 94-.
[8]	Ruijie Li, Mengmeng Zhou, Shilong He, Tingting Pan, Jing Liu, Jiabao Zhu. Deciphering the effect of sodium dodecylbenzene sulfonate on up-flow anaerobic sludge blanket treatment of synthetic sulfate-containing wastewater[J]. Front. Environ. Sci. Eng., 2021, 15(5): 91-.
[9]	Weichuan Qiao, Rong Li, Tianhao Tang, Achuo Anitta Zuh. Removal, distribution and plant uptake of perfluorooctane sulfonate (PFOS) in a simulated constructed wetland system[J]. Front. Environ. Sci. Eng., 2021, 15(2): 20-.
[10]	Yanqing Duan, Aijuan Zhou, Xiuping Yue, Zhichun Zhang, Yanjuan Gao, Yanhong Luo, Xiao Zhang. Acceleration of the particulate organic matter hydrolysis by start-up stage recovery and its original microbial mechanism[J]. Front. Environ. Sci. Eng., 2021, 15(1): 12-.
[11]	Yuhan Zheng, Zhiguo Su, Tianjiao Dai, Feifei Li, Bei Huang, Qinglin Mu, Chuanping Feng, Donghui Wen. Identifying human-induced influence on microbial community: A comparative study in the effluent-receiving areas in Hangzhou Bay[J]. Front. Environ. Sci. Eng., 2019, 13(6): 90-.
[12]	Aoshuang Jing, Tao Liu, Xie Quan, Shuo Chen, Yaobin Zhang. Enhanced nitrification in integrated floating fixed-film activated sludge (IFFAS) system using novel clinoptilolite composite carrier[J]. Front. Environ. Sci. Eng., 2019, 13(5): 69-.
[13]	Qinqin Liu, Miao Li, Rui Liu, Quan Zhang, Di Wu, Danni Zhu, Xuhui Shen, Chuanping Feng, Fawang Zhang, Xiang Liu. Removal of trimethoprim and sulfamethoxazole in artificial composite soil treatment systems and diversity of microbial communities[J]. Front. Environ. Sci. Eng., 2019, 13(2): 28-.
[14]	Xiaohui Wang, Shuai Du, Tao Ya, Zhiqiang Shen, Jing Dong, Xiaobiao Zhu. Removal of tetrachlorobisphenol A and the effects on bacterial communities in a hybrid sequencing biofilm batch reactor-constructed wetland system[J]. Front. Environ. Sci. Eng., 2019, 13(1): 14-.
[15]	Yonglei Wang, Baozhen Liu, Kefeng Zhang, Yongjian Liu, Xuexin Xu, Junqi Jia. Investigate of in situ sludge reduction in sequencing batch biofilm reactor: Performances, mechanisms and comparison of different carriers[J]. Front. Environ. Sci. Eng., 2018, 12(5): 5-.

Viewed

Full text

Abstract

Cited

Shared

Discussed