Proliferation of osteoblast cells on nanotubes

doi:10.1007/s11706-009-0034-z

Front Mater Sci Chin

2009, Vol. 3

Issue (2) : 169-173 https://doi.org/10.1007/s11706-009-0034-z

RESEARCH ARTICLE

Proliferation of osteoblast cells on nanotubes

F. WATARI(

), T. AKASAKA, Xiaoming LI, M. UO, A. YOKOYAMA

Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan

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Abstract

Carbon nanotubes (CNT) have a unique structure and feature. In the present study, cell proliferation was performed on the scaffolds of single-walled CNTs (SWCNT), multiwalled CNTs (MWCNT), and on graphite, one of the representative isomorphs of pure carbon, for the sake of comparison. Scanning electron microscopy observation of the growth of osteoblast-like cells (Saos2) cultured on CNTs showed the morphology fully developed for the whole direction, which is different from that extended to one direction on the usual scaffold. Numerous filopodia were grown from cell edge, extended far long and combined with the CNT meshwork. CNTs showed the affinity for collagen and proteins. Proliferated cell numbers are largest on SWCNTs, followed by MWCNTs, and are very low on graphite. This is in good agreement with the sequence in the results of the adsorbed amount of proteins and expression of alkaline phosphatase activity for these scaffolds. The adsorption of proteins would be one of the most influential factors to make a contrast difference in cell attachment and proliferation between graphite and CNTs, both of which are isomorphs of carbon and composed of similar graphene sheet crystal structure. In addition, the nanosize meshwork structure with large porosity is another property responsible for the excellent cell adhesion and growth on CNTs. CNTs could be the favorable materials for biomedical applications.

Keywords carbon nanotube scaffold osteoblast regeneration nanomaterial

Corresponding Author(s): WATARI F.,Email:watari@den.hokudai.ac.jp

Issue Date: 05 June 2009

Cite this article:

F. WATARI,T. AKASAKA,Xiaoming LI, et al. Proliferation of osteoblast cells on nanotubes[J]. Front Mater Sci Chin, 2009, 3(2): 169-173.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-009-0034-z
https://academic.hep.com.cn/foms/EN/Y2009/V3/I2/169

Fig.1 Schematic figures of two different crystal structures of carbon graphite and CNT

Fig.2 SEM image of osteoblast-like Saos2 cells cultured on graphite and MWCNT scaffolds for 7 d

Fig.3 Number of Saos2 cells cultured on the scaffolds of polycarbonate, graphite, MWCNT, and SWCNT for 3 and 7 d []

Fig.4 SEM observation of filopodia grown from the periphery of osteoblast-like cells on polycarbonate and MWCNT scaffolds

Fig.5 SEM image of CNTs after immersion in SBF for 2 weeks

Fig.6 SEM image of dentin surface etched with phosphoric acid after being immersed in CNT mixed solution

Fig.7 Adsorbed amount of proteins on the scaffolds of polycarbonate (PC), graphite (GP), MWCNT (MW), and SWCNT (SW) in cell culture medium after 24 h []

Fig.8 Expression of total ALP activity from Saos2 cells cultured on the scaffolds of polycarbonate (PC), graphite (GP), MWCNT(MW), and SWCNT (SW)for 3 and 7 d []

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