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Durability of neutralizing antibodies and T-cell response post SARS-CoV-2 infection |
Yun Tan1, Feng Liu1, Xiaoguang Xu1, Yun Ling2, Weijin Huang3, Zhaoqin Zhu2, Mingquan Guo2, Yixiao Lin2, Ziyu Fu1, Dongguo Liang1, Tengfei Zhang2, Jian Fan2, Miao Xu3, Hongzhou Lu2(), Saijuan Chen1() |
1. Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 2. Shanghai Public Health Clinical Center, Shanghai 201508, China 3. National Institutes for Food and Drug Control (NIFDC), Beijing 102629, China |
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Abstract The ongoing pandemic of coronavirus disease 19 (COVID-19) is caused by a newly discovered β coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). How long the adaptive immunity triggered by SARS-CoV-2 can last is of critical clinical relevance in assessing the probability of second infection and efficacy of vaccination. Here we examined, using ELISA, the IgG antibodies in serum specimens collected from 17 COVID-19 patients at 6–7 months after diagnosis and the results were compared to those from cases investigated 2 weeks to 2 months post-infection. All samples were positive for IgGs against the S- and N-proteins of SARS-CoV-2. Notably, 14 samples available at 6–7 months post-infection all showed significant neutralizing activities in a pseudovirus assay, with no difference in blocking the cell-entry of the 614D and 614G variants of SARS-CoV-2. Furthermore, in 10 blood samples from cases at 6–7 months post-infection used for memory T-cell tests, we found that interferon γ-producing CD4+ and CD8+ cells were increased upon SARS-CoV-2 antigen stimulation. Together, these results indicate that durable anti-SARS-CoV-2 immunity is common in convalescent population, and vaccines developed from 614D variant may offer protection from the currently predominant 614D variant of SARS-CoV-2.
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Keywords
SARS-CoV-2
neutralizing antibodies
T-cell response
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Corresponding Author(s):
Hongzhou Lu,Saijuan Chen
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Just Accepted Date: 16 September 2020
Online First Date: 30 September 2020
Issue Date: 24 December 2020
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1 |
G Zhou, S Chen, Z Chen. Back to the spring of 2020: facts and hope of COVID-19 outbreak. Front Med 2020; 14(2): 113–116
https://doi.org/10.1007/s11684-020-0758-9
pmid: 32172487
|
2 |
G Zhou, S Chen, Z Chen. Advances in COVID-19: the virus, the pathogenesis, and evidence-based control and therapeutic strategies. Front Med 2020; 14(2): 117–125
https://doi.org/10.1007/s11684-020-0773-x
pmid: 32318975
|
3 |
M Zhou, X Zhang, J Qu. Coronavirus disease 2019 (COVID-19): a clinical update. Front Med 2020; 14(2): 126–135
https://doi.org/10.1007/s11684-020-0767-8
pmid: 32240462
|
4 |
N Le Bert, AT Tan, K Kunasegaran, CYL Tham, M Hafezi, A Chia, MHY Chng, M Lin, N Tan, M Linster, WN Chia, MI Chen, LF Wang, EE Ooi, S Kalimuddin, PA Tambyah, JG Low, YJ Tan, A Bertoletti. SARS-CoV-2-specific T cell immunity in cases of COVID-19 and SARS, and uninfected controls. Nature 2020; 584(7821): 457–462
https://doi.org/10.1038/s41586-020-2550-z
pmid: 32668444
|
5 |
A Grifoni, D Weiskopf, SI Ramirez, J Mateus, JM Dan, CR Moderbacher, SA Rawlings, A Sutherland, L Premkumar, RS Jadi, D Marrama, AM de Silva, A Frazier, AF Carlin, JA Greenbaum, B Peters, F Krammer, DM Smith, S Crotty, A Sette. Targets of T cell responses to SARS-CoV-2 coronavirus in humans with COVID-19 disease and unexposed individuals. Cell 2020; 181(7): 1489–1501.e15
https://doi.org/DOI: 10.1016/j.cell.2020.05.015
pmid: 32473127
|
6 |
KK To, OT Tsang, WS Leung, AR Tam, TC Wu, DC Lung, CC Yip, JP Cai, JM Chan, TS Chik, DP Lau, CY Choi, LL Chen, WM Chan, KH Chan, JD Ip, AC Ng, RW Poon, CT Luo, VC Cheng, JF Chan, IF Hung, Z Chen, H Chen, KY Yuen. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect Dis 2020; 20(5): 565–574
https://doi.org/10.1016/S1473-3099(20)30196-1
pmid: 32213337
|
7 |
DF Robbiani, C Gaebler, F Muecksch, JCC Lorenzi, Z Wang, A Cho, M Agudelo, CO Barnes, A Gazumyan, S Finkin, T Hägglöf, TY Oliveira, C Viant, A Hurley, HH Hoffmann, KG Millard, RG Kost, M Cipolla, K Gordon, F Bianchini, ST Chen, V Ramos, R Patel, J Dizon, I Shimeliovich, P Mendoza, H Hartweger, L Nogueira, M Pack, J Horowitz, F Schmidt, Y Weisblum, E Michailidis, AW Ashbrook, E Waltari, JE Pak, KE Huey-Tubman, N Koranda, PR Hoffman, AP West Jr, CM Rice, T Hatziioannou, PJ Bjorkman, PD Bieniasz, M Caskey, MC Nussenzweig. Convergent antibody responses to SARS-CoV-2 in convalescent individuals. Nature 2020; 584(7821): 437–442
https://doi.org/10.1038/s41586-020-2456-9
pmid: 32555388
|
8 |
FJ Ibarrondo, JA Fulcher, D Goodman-Meza, J Elliott, C Hofmann, MA Hausner, KG Ferbas, NH Tobin, GM Aldrovandi, OO Yang. Rapid decay of anti-SARS-CoV-2 antibodies in persons with mild Covid-19. N Engl J Med 2020; 383(11): 1085–1087
https://doi.org/10.1056/NEJMc2025179
pmid: 32706954
|
9 |
DF Gudbjartsson, GL Norddahl, P Melsted, K Gunnarsdottir, H Holm, E Eythorsson, AO Arnthorsson, D Helgason, K Bjarnadottir, RF Ingvarsson, B Thorsteinsdottir, S Kristjansdottir, K Birgisdottir, AM Kristinsdottir, MI Sigurdsson, GA Arnadottir, EV Ivarsdottir, M Andresdottir, F Jonsson, AB Agustsdottir, J Berglund, B Eiriksdottir, R Fridriksdottir, EE Gardarsdottir, M Gottfredsson, OS Gretarsdottir, S Gudmundsdottir, KR Gudmundsson, TR Gunnarsdottir, A Gylfason, A Helgason, BO Jensson, A Jonasdottir, H Jonsson, T Kristjansson, KG Kristinsson, DN Magnusdottir, OT Magnusson, LB Olafsdottir, S Rognvaldsson, L le Roux, G Sigmundsdottir, A Sigurdsson, G Sveinbjornsson, KE Sveinsdottir, M Sveinsdottir, EA Thorarensen, B Thorbjornsson, M Thordardottir, J Saemundsdottir, SH Kristjansson, KS Josefsdottir, G Masson, G Georgsson, M Kristjansson, A Moller, R Palsson, T Gudnason, U Thorsteinsdottir, I Jonsdottir, P Sulem, K Stefansson. Humoral immune response to SARS-CoV-2 in Iceland. N Engl J Med 2020; [Epub ahead of print] doi: 10.1056/NEJMoa2026116
https://doi.org/10.1056/NEJMoa2026116
pmid: 32871063
|
10 |
G Alter, R Seder. The power of antibody-based surveillance. N Engl J Med 2020; [Epub ahead of print] doi: 10.1056/NEJMe2028079
https://doi.org/10.1056/NEJMe2028079
pmid: 32871061
|
11 |
NN Zhang, XF Li, YQ Deng, H Zhao, YJ Huang, G Yang, WJ Huang, P Gao, C Zhou, RR Zhang, Y Guo, SH Sun, H Fan, SL Zu, Q Chen, Q He, TS Cao, XY Huang, HY Qiu, JH Nie, Y Jiang, HY Yan, Q Ye, X Zhong, XL Xue, ZY Zha, D Zhou, X Yang, YC Wang, B Ying, CF Qin. A thermostable mRNA vaccine against COVID-19. Cell 2020; 182(5): 1271–1283.e16
https://doi.org/DOI: 10.1016/j.cell.2020.07.024
pmid: 32795413
|
12 |
J Nie, Q Li, J Wu, C Zhao, H Hao, H Liu, L Zhang, L Nie, H Qin, M Wang, Q Lu, X Li, Q Sun, J Liu, C Fan, W Huang, M Xu, Y Wang. Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2. Emerg Microbes Infect 2020; 9(1): 680–686
https://doi.org/10.1080/22221751.2020.1743767
pmid: 32207377
|
13 |
H Shi, J Ye, J Teng, Y Yin, Q Hu, X Wu, H Liu, X Cheng, Y Su, M Liu, J Gu, T Lu, H Chen, H Zheng, Y Sun, C Yang. Elevated serum autoantibodies against co-inhibitory PD-1 facilitate T cell proliferation and correlate with disease activity in new-onset systemic lupus erythematosus patients. Arthritis Res Ther 2017; 19(1): 52
https://doi.org/10.1186/s13075-017-1258-4
pmid: 28274252
|
14 |
C Goudot, A Coillard, AC Villani, P Gueguen, A Cros, S Sarkizova, TL Tang-Huau, M Bohec, S Baulande, N Hacohen, S Amigorena, E Segura. Aryl hydrocarbon receptor controls monocyte differentiation into dendritic cells versus macrophages. Immunity 2017; 47(3): 582–596.e6
https://doi.org/DOI: 10.1016/j.immuni.2017.08.016
pmid: 28930664
|
15 |
B Korber, WM Fischer, S Gnanakaran, H Yoon, J Theiler, W Abfalterer, N Hengartner, EE Giorgi, T Bhattacharya, B Foley, KM Hastie, MD Parker, DG Partridge, CM Evans, TM Freeman, TI de Silva, C-GG Sheffield, C McDanal, LG Perez, H Tang, A Moon-Walker, SP Whelan, CC LaBranche, EO Saphire, DC Montefiori. Tracking changes in SARS-CoV-2 spike: evidence that D614G increases infectivity of the COVID-19 virus. Cell 2020; 182(4): 812–827.e19
https://doi.org/DOI: 10.1016/j.cell.2020.06.043
pmid: PMID: 32697968
|
16 |
X Zhang, Y Tan, Y Ling, G Lu, F Liu, Z Yi, X Jia, M Wu, B Shi, S Xu, J Chen, W Wang, B Chen, L Jiang, S Yu, J Lu, J Wang, M Xu, Z Yuan, Q Zhang, X Zhang, G Zhao, S Wang, S Chen, H Lu. Viral and host factors related to the clinical outcome of COVID-19. Nature 2020; 583(7816): 437–440
https://doi.org/10.1038/s41586-020-2355-0
pmid: 32434211
|
17 |
TF Rogers, F Zhao, D Huang, N Beutler, A Burns, WT He, O Limbo, C Smith, G Song, J Woehl, L Yang, RK Abbott, S Callaghan, E Garcia, J Hurtado, M Parren, L Peng, S Ramirez, J Ricketts, MJ Ricciardi, SA Rawlings, NC Wu, M Yuan, DM Smith, D Nemazee, JR Teijaro, JE Voss, IA Wilson, R Andrabi, B Briney, E Landais, D Sok, JG Jardine, DR Burton. Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model. Science 2020; 369(6506): 956–963
https://doi.org/10.1126/science.abc7520
pmid: 32540903
|
18 |
H Peng, LT Yang, J Li, ZQ Lu, LY Wang, RA Koup, RT Bailer, CY Wu. Human memory T cell responses to SARS-CoV E protein. Microbes Infect 2006; 8(9-10): 2424–2431
https://doi.org/10.1016/j.micinf.2006.05.008
pmid: 16844400
|
19 |
H Peng, LT Yang, LY Wang, J Li, J Huang, ZQ Lu, RA Koup, RT Bailer, CY Wu. Long-lived memory T lymphocyte responses against SARS coronavirus nucleocapsid protein in SARS-recovered patients. Virology 2006; 351(2): 466–475
https://doi.org/10.1016/j.virol.2006.03.036
pmid: 16690096
|
20 |
OW Ng, A Chia, AT Tan, RS Jadi, HN Leong, A Bertoletti, YJ Tan. Memory T cell responses targeting the SARS coronavirus persist up to 11 years post-infection. Vaccine 2016; 34(17): 2008–2014
https://doi.org/10.1016/j.vaccine.2016.02.063
pmid: 26954467
|
21 |
L Yang, H Peng, Z Zhu, G Li, Z Huang, Z Zhao, RA Koup, RT Bailer, C Wu. Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen. J Gen Virol 2007; 88(Pt 10): 2740–2748
https://doi.org/10.1099/vir.0.82839-0
pmid: 17872527
|
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