Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral blood

doi:10.15302/J-FASE-2017162

Front. Agr. Sci. Eng.

2017, Vol. 4

Issue (3) : 342-352 https://doi.org/10.15302/J-FASE-2017162

RESEARCH ARTICLE

Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral blood

Haiyan WANG^1,^2,³, Qiaoxia ZHANG^1,², Lilin YIN^1,², Xiangdong LIU^1,², Shuhong ZHAO^1,², Mengjin ZHU^1,², Changchun LI^1,²(

)

¹. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
². The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China
³. College of Informatics, Huazhong Agricultural University, Wuhan 430070, China

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Abstract

Neutrophils are vital components of defense mechanisms against invading pathogens and are closely linked with the individual antiviral capacity of pigs and other mammals. Neutrophilia is a well-known clinical characteristic of viral and bacterial infections. Using Affymetrix porcine genome microarrays, we investigated the gene expression profiles associated with neutrophil variation in porcine peripheral blood before and after polyriboinosinic-polyribocytidylic acid stimulation. Transcriptomic analysis showed 796 differentially expressed genes (DEGs) in extreme response (ER) pigs and 192 DEGs in moderate response (MR) pigs. Most DEGs were related to immune responses, included MXD1, CXCR4, CREG1, MyD88, CD14, TLR2, TLR4, IRF3 and IRF7. Gene ontology analysis indicated that the DEGs of both ER and MR pigs were involved in common biological processes, such as cell proliferation, growth regulation, immune response, inflammatory response and cell activation. The ER and MR groups also showed differences in DEGs involved in biological processes. DEGs involved in cell division and cell cycle were specifically found in the ER pigs, whereas DEGs involved in cell migration were specifically found in the MR pigs. The study provides a basic understanding of the molecular basis for the antiviral capacity of pigs and other mammals.

Keywords neutrophil peripheral blood pig poly I:C transcriptome

Corresponding Author(s): Changchun LI

Just Accepted Date: 27 May 2017 Online First Date: 21 June 2017 Issue Date: 12 September 2017

Cite this article:

Haiyan WANG,Qiaoxia ZHANG,Lilin YIN, et al. Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral blood[J]. Front. Agr. Sci. Eng. , 2017, 4(3): 342-352.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2017162
https://academic.hep.com.cn/fase/EN/Y2017/V4/I3/342

Fig.1 Increase in neutrophil ratio induced by polyriboinosinic-polyribocytidylic acid. The black and white bars indicate the average increase in neutrophil ratio in the extreme and moderate response pigs (ER and MR) after poly I:C stimulation, respectively. The difference between two groups of pigs was significant (P<0.01).

Fig.2 Hierarchical cluster analysis of all samples. (a) Cluster of unstimulated and poly I:C-stimulated conditions in extreme response (ER) pigs; (b) cluster of unstimulated and poly I:C-stimulated conditions in the moderate response (MR) pigs. The figure depicts high (red) and low (green) relative levels of gene expression. Mean-centered gene expression ratios are depicted by a log2 pseudocolor scale (ratio fold change indicated); gray denotes poorly measured data.

Fig.3 Venn diagram of differentially expressed genes in the extreme and moderate response pigs (ER and MR)

Fig.4 Validation of microarray data by real-time qRT-PCR analyses. (a) Six genes were selected for qRT-PCR experiments in extreme response (ER) pigs; (b) expression of ten genes in moderate response (MR) pigs was measured by qRT-PCR experiments. The black and gray bars show gene expression before and after poly I:C stimulation. * denotes statistically significant differences (P<0.05).

Fig.5 Comparative functional annotation of differentially expressed genes (DEGs). DEGs were grouped according to their putative functions. (a) Comparison of distribution of the DEGs by Gene Ontology (GO) biological process induced by poly I:C treatment in the extreme and moderate response pigs (ER and MR); (b) comparison of distribution of the DEGs by GO cellular component induced by poly I:C treatment in the ER and MR pigs; (c) comparative GO molecular function distribution of the DEGs following poly I:C treatment of the ER and MR pigs; (d) comparative KEGG pathway annotation distribution of the DEGs following poly I:C treatment in the ER and MR pigs. The black and white bars indicate the number of altered genes in the ER and MR groups, respectively.

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